The spectrophotometric titration of the tyrosine residues,
their reaction with N-acetylimidazole, and the effect of pH on the
thermally induced conformational transition of ribonuclease T₁ have
been investigated. The results of spectrophotometric titration in
0.20 M KCl/0.001 M sodium phosphate suggest that titration of the
tyrosine residues occurs in two...
The thermophilic aldolase from Bacillus stearothermophilus
(NCA 2184) has been purified to a specific activity of 45.5 μ-moles
of fructose-1, 6-diphosphate cleaved per minute per mg of enzyme,
representing a 624-fold increase over the crude extract. The preparation appeared to be homogenous by sedimentation velocity ultra-centrifugation
and by a specific...
This study was undertaken to determine the sorts of polygalacturonases
present in the culture filtrate of Fusarium oxysporum
f. sp. lycopersici, to purify and characterize these enzymes, and to
study any complexes that may form between polygalacturonase and
carbohydrate not degraded by the enzyme.
The polygalacturonase in culture filtrate consists...
An Ls-isocitrate:NADP oxidoreductase has been isolated and
purified from embryonic chicken liver. The enzyme was purified
200 fold by means of ammonium sulfate fractionation, gel filtration
chromatography on Sephadex G-200, ion-exchange chromatography
on Bio-Gel CM-100 and adsorption chromatography on hydroxylapatite
gel.
The purified enzyme was nearly homogeneous as determined
from...