The objective of this study was to examine optimum conditions for primary
culture of porcine preadipocytes. Dorsal subcutaneous adipose tissue was obtained
from biopsies of 40-50 kg pigs and digested with collagenase in Krebs Ringer
Bicarbonate buffer. Isolated stromal vascular cells were cultured in different test media.
Stromal vascular cells...
The accumulation of excessive fat is a serious concern in both
the livestock production and human health fields. Obesity is a
condition of excessive energy storage in the form of body fat
( triacylglycerols ). The cellular basis for obesity is not yet
understood but numerous factors have been suggested....
In recent years, the general public has recognized the dangers of a high fat diet and
are demanding meat with lower fat content. This demand has stimulated research in the
growth and regulation of adipocytes. However, despite much effort, no adipocyte-specific
plasma membrane markers from any species are available as...