Investigations with ram semen were conducted to develop procedures
of cyrogenic preservation that would provide long-term
retention of fertilizing capacity in ram spermatozoa.
An isotonic extender consisting of egg yolk, sodium citrate,
lactose, glycerol, and a broad spectrum antibiotic was selected from
among several extenders tested in pilot studies. Using...
Successful fertilization of fresh steelhead trout, pink salmon, kokanee, chum salmon, fall chinook salmon, and coho salmon eggs with cryopreserved sperm is reported in this thesis. A mean percent fertilization greater than 50 percent was achieved for all species tested except chum salmon. Differences in sperm viability from donor males...
