Developing sea urchin eggs (Strongylocentrotus purpuratus),
reared at 15° C, were subjected to single 15-minute treatments with
disodium versenate in calcium-free sea water, applied at 10-minute
intervals, from 4 minutes after insemination to the second cleavage.
These versene pulses produced specific degrees of retardation in
the mitotic process and delayed...
Accurate chromosome segregation and cell cleavage are critical to maintaining genomic integrity. Both events involve the spindle apparatus, but the exact mechanics is as puzzling as the contradicting models proposed in the last two centuries. In this dissertation, current prevailing models of chromosome segregation and cell cleavage are tested using...