Preparation of a cytochrome c-cytochrome oxidase complex
was achieved, using crystalline beef cytochrome c and purified
cytochrome oxidase from beef heart muscle, or from the
Keilin-Hartree preparation. Succinate oxidase was reconstituted
with the complex.
A mixture of cytochrome oxidase, solubilized with nonionic
detergent, and excess cytochrome c was exposed to...
The spectrophotometric method for the determination of the
acid nonextractable flavin in the proteolytic digest of the trichloroacetic
acid precipitate from the heart muscle preparation has been
examined for the interference of the degraded hemeproteins. The
proteolytic digest was chromatographically separated into the heme
and flavin fractions. Spectrophotometric determinations made...
NADPH-cytochrome P-450 reductase, an important enzyme in the
cytochrome P-450-dependent microsomal monooxygenase system, was
purified from insecticide-resistant (R) and -susceptible (S) house
flies (Musca domestica, L.) and from the black blow fly (Phormia
regina, Weigen). Proteolytic degradation to a P-450 - inactive form
of the reductase was reduced during the...
Coproporphyrinogen III oxidase (CPX) is an enzyme involved in the biosynthesis
of tetrapyrroles, catalyzing the oxidative decarboxylation of coproporphyrinogen III to
protoporphyrinogen IX. In bacteria and yeast this enzyme is cytosolic, in animals it is
mitochondrial, and in each it functions to produce heme. However, in all plants
investigated, the...
Polyphenol oxidase (PPO) from d'Anjou pears was purified and some
molecular properties were studied. The extraction of PPO was
accomplished in the presence of the phenolic binder AG 2X-8 and Triton
X-100. Chlorophyll pigment was removed by chromatography resulting in
a clear, colorless enzyme extract. Three fractions of pear PPO...