Cell culture systems have provided many insights into
eukaryotic gene expression and other biochemical
mechanisms. Since the cell represents the smallest living
unit of any organism it provides a desirable in vitro
system, allowing biochemical studies without the complex
physiology of an entire animal. However, processes
involving intracellular mechanisms, such...
Rat selenoprotein W (SeW) promoter activity was investigated using different concentrations of cadmium, copper, and zinc. Two fragments (404bp and 1265bp) of the SeW promoter, containing a single metal response element (MRE), were ligated into the multiple cloning site of a pGL3-Basic reporter plasmid. The constructs were transfected into cultured...