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Filtering by: Subject Proteins -- Crosslinking

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Creator:

Jensen, Ole Norregaard

Abstract:

A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated to be feasible. The protocol combines photochemical crosslinking techniques and mass spectrometric methods into a new strategy for identifying protein domains or amino acid residues that are in close contact with nucleic acid in protein-nucleic acid...

Resource Type:

Dissertation
Characterization of UV-crosslinked protein-nucleic acid interfaces by Maldi MS and ESI MS/MS

Creator:

Gafken, Philip R.

Resource Type:

Dissertation
Mass spectrometric analysis of UV-crosslinked protein-nucleic acid complexes

Creator:

Doneanu, Catalin E.

Abstract:

The DNA-binding domains of E. coli uracil-DNA glycosylase (Ung) and human replication protein A (hRPA) were studied using a general protocol developed in our laboratory for probing protein-DNA interactions. The procedure involves purification and mass spectrometric analysis of the nucleopeptide-products of a tryptically digested UV-crosslinked protein-nucleic acid complex. In the...

Resource Type:

Dissertation