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Thermally induced leakage and viability studies in an obligate psychrophilic marine bacterium

Creator:: Kenis, Paul Robert
Abstract:: Vibrio marinus MP-1, an obligate psychrophilic marine bacterium, was severely damaged when heat-shocked in the presence of nutrients. Thermally induced leakage materials from cells tested for in the medium were 260 mμ absorbing material (nucleic acids), orcinol reacting material (RNA), ninhydrin reacting material (amino acids), protein, malic dehydrogenase, and glucose-6-phosphate...
Resource Type:: Masters Thesis

Thermal inactivation studies on some enzymes from Vibrio marinus, an obligately psychrophilic marine bacterium

Creator:: Mathemeier, Paul Fredrick
Abstract:: Some enzymes of the citric acid cycle and glycolytic pathway in cell-free extracts of Vibrio marinus MP-1 were compared for thermal lability. After one hour of moderate temperature exposure, enzymes of both pathways rapidly lost catalytic activity. For all but one enzyme, 50 percent remaining activity occurred near an averaged...
Resource Type:: Dissertation

The effect of temperature and hydrostatic pressure on protein, ribonucleic acid and deoxytibo-nucleic acid synthesis by Vibrio marinus, an obligate psychrophile

Creator:: Albright, Lawrence John
Abstract:: The marine psychrophilic bacterium Vibrio marinus MP-4 possessed a maximum temperature and hydrostatic pressure of 20 C and 425 atm for growth. The effects of temperatures of 21 and 25 C and hydrostatic pressures of 200, 400, 500, and 1,000 atm on protein, RNA and DNA synthesis by V. marinus...
Resource Type:: Dissertation

Glucose uptake and catabolism in the marine psychrophilic bacterium, Vibrio marinus

Creator:: Griffiths, Robert Porter
Abstract:: The effects of various physical parameters on the binding, uptake, and catabolism of glucose in the marine psychrophilic bacterium, Vibrio marinus, were studied. It was shown that shortly after the cell was exposed to labeled glucose, the radioactive label became rapidly associated with the cell fraction. It was also shown...
Resource Type:: Dissertation

The effects of hydrostatic pressure and temperature on the uptake and utilization of amino acids by a facultatively psychrophilic marine bacterium

Creator:: Paul, Kala Lapidus
Abstract:: The transport response of four marine psychrophilic bacteria to a variety of substrates was determined and related to environmental conditions. Studies on pressure and temperature effects on glutamic acid transport and utilization indicated that high pressures and low temperatures inhibit glutamate transport, while permitting glutamate respiration. Similar results were obtained...
Resource Type:: Masters Thesis

Effects of salinity and temperature changes on the induction of glutamic dehydrogenase in the marine psychrophilic bacterium, Vibrio marinus MP-1

Creator:: Staley, Thomas Edwin
Abstract:: In this study the relationship between salinity and temperature changes on microbial growth, enzyme induction and substrate uptake were investigated. The obligately psychrophilic bacterium, V. marinus MP-1 was grown for 48 hours at 15 C in a glucose-ammonium medium (GAM) containing 0.4 M NaCl. Log-phase cells were harvested and shifted...
Resource Type:: Dissertation

Some physiological effects of near maximum growth temperatures on an obligately psychrophilic marine bacterium

Creator:: Geesey, G. G.
Abstract:: The heat inactivation of the psychrophilic marine bacterium, Ant-300, was investigated in terms of permeability control, glucose uptake, and respiration. Extensive leakage of 14C-cellular material occurred at 13 C, the maximum growth temperature of the organism. Protein and RNA were released from the cells in significant quantities. Inhibition of glucose...
Resource Type:: Masters Thesis

Substrate capture by a psychrophilic marine bacterium

Creator:: Geesey, G. G.
Abstract:: Cells of the marine bacterium, Ant-300, accumulate loosely bound amino acids during amino acid uptake. The fraction of amino acid taken up that exists in the loosely bound state depends on the substrate in question. Shock treatment studies indicate that, in general, the initial binding, uptake, and retention of amino...
Resource Type:: Dissertation

The synergetic effects of starvation and hydrostatic pressure on uptake of alpha-aminoisobutyric acid by a psychrophilic marine vibrio

Creator:: Yorgey, Peter Scott
Resource Type:: Masters Thesis

Growth rate effects during starvation-survival of a marine psychrophilic vibrio

Creator:: Moyer, Craig Lee
Abstract:: Cell populations of the marine bacterium ANT-300, from either batch culture or continuous culture with dilution rates ranging from D = 0.170 h⁻¹ (fast) to D = 0.015 h⁻¹ (slow) were monitored for 98 days under starvation. Viability (CFU), acridine orange direct counts (AODC), and optical density were measured. DNA,...
Resource Type:: Masters Thesis

ScholarsArchive@OSU

Thermally induced leakage and viability studies in an obligate psychrophilic marine bacterium

Thermal inactivation studies on some enzymes from Vibrio marinus, an obligately psychrophilic marine bacterium

The effect of temperature and hydrostatic pressure on protein, ribonucleic acid and deoxytibo-nucleic acid synthesis by Vibrio marinus, an obligate psychrophile

Glucose uptake and catabolism in the marine psychrophilic bacterium, Vibrio marinus

The effects of hydrostatic pressure and temperature on the uptake and utilization of amino acids by a facultatively psychrophilic marine bacterium

Effects of salinity and temperature changes on the induction of glutamic dehydrogenase in the marine psychrophilic bacterium, Vibrio marinus MP-1

Some physiological effects of near maximum growth temperatures on an obligately psychrophilic marine bacterium

Substrate capture by a psychrophilic marine bacterium

The synergetic effects of starvation and hydrostatic pressure on uptake of alpha-aminoisobutyric acid by a psychrophilic marine vibrio

Growth rate effects during starvation-survival of a marine psychrophilic vibrio

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