The rainbow trout (Oncorhynchus mykiss) model
of chemical carcinogenesis is becoming increasingly
important as a supplement to rodent studies. However,
much of the molecular biology of the carcinogenic
response is still unknown in the trout model. The ras
gene family has been implicated in the tumorigenesis of
both spontaneous and...
Rainbow trout were exposed to aluminum at pH 7.25 and
8.25 and four hardnesses (10, 30, 80, and 120 ppm CaCO₃)
for 96 hours in a continuous-flow system and mortality and
aluminum accumulation in the gills were determined.
Temperature, pH, and dissolved oxygen were measured
daily for each treatment. Dissolved...
For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
Of 109 aquatic birnaviruses (AB) belonging to 9 serogroup A, all replicated and produced rapid and extensive cytopathology in CHSE-214 and RTG-2 cells, whereas only half produced significant levels of cytopathological effect (CPE) in two nonsalmonid cell lines tested (EPC and FHM). In many instances it was found that although...
Temperature influences the incidence of chemically induced cancer in fish,
with warmer temperatures being associated with higher cancer incidence. The
mechanisms of temperature-modulated chemical carcinogenesis in fish, however,
have not been described in detail. Therefore, one primary objective of this study
was increased understanding of how temperature-modulated genotoxicity of 7,12-...
The characteristics of an inhibitor of infectious pancreatic necrosis virus (IPNV)
found in normal rainbow trout serum (RTS) were studied. The serum inhibitor had a
molecular weight of approximately 150 kDa and was dependent on divalent cations,
either Ca²⁺ or Mg²⁺. It was stable at temperatures up to 50°C and...
The hypotheses to be tested in this investigation are: 1) there is a threshold level of liver lipid and glycogen content above which the health condition of hatchery-reared fish is impaired, 2) fatty and high glycogen livers impair liver function and structure, and 3) fatty and high glycogen livers increase...
Clinical methods to diagnose specific organ dysfunction have not been evaluated in fishes. To determine if an accepted clinical test of mammalian liver function could be applied to fishes, the
hepatic disposition of the organic anion sulfobromophthalein (BSP) was studied in rainbow trout (Salmo gairdneri) treated with a model hepatotoxicant....