Four cell lines derived from embryonic tissues of four species
of salmonid fishes, and one from a hepatoma in rainbow trout have
been established in this laboratory. The species represented are
chinook, coho, and sockeye salmon; and steelhead and rainbow trout.
All of these lines have been cultivated for approximately...
A new Lactobacillus species is proposed to include 17 strains of a bacterium from rainbow (Salmo gairdneri) and cutthroat trout (Salmo clarki), and chinook salmon (Oncorhynchus tshawytscha). The bacterium was isolated most frequently from fish stressed by handling or spawn ing. Phenotypically, the strains belong to the family Lactobacillaceae and...
Salmonid B lymphocytes were stimulated both in vivo and in vitro
and shown to produce antibody specific for the immunogen. A passive
hemolytic plaque assay was employed to demonstrate B cells producing
specific antibody. Characterization of plaque forming cells (PFCs)
involved sequential inhibition with a series of graded concentrations
of...
Sexual maturation and sex differentiation comprise facets of a
common theme: reproduction. The endocrine system regulates many
of the critical physiological processes necessary for reproduction and
offers a framework within which technologies can be developed for
controlling sexual maturation and sex differentiation. The studies
described in this thesis were undertaken...
The foundation of the research compilation presented here began
with the derivation of an improved method for obtaining optimal in vitro
mitogenic responses in salmonid lymphocyte cultures by utilizing
autologous or homologous plasma as the primary serum supplement. It was
observed that lymphocytes which were previously unresponsive to
mitogenic challenge...
Genetic, ontogenetic, and environmental factors modified characteristic interrenal and glycemic responses to stress in juvenile salmonid fishes. During continuous confinement stress, plasma cortisol rose more quickly in chinook salmon, Oncorhynchus tshawytscha, acclimated to high rather than medium or low temperatures; hyperglycemia following either acute or chronic stress was also highest...
A theory of the origin and distribution of fishes in the Harney Basin is presented. Blitzen River, the hydrographically and ecologically isolated creeks, and the upland tributaries of Silvies River all contain populations derived from the basin's Pleistocene connection with Malheur River of the Snake system. Colonization of many streams...
The behavior of two species of stream salmonids, juvenile coho salmon, Oncorhynchus kisutch (Walbaurn) and cutthroat trout, Salmo clarki clarki Richardson, was studied in terms of the time spent by individual fish in carrying out various activities. Studies were conducted from June 1968, through May 1969, at Berry Creek, a...