The occurrence and distribution of fish viruses in Oregon were
determined by the examination of anadromous and fresh water salmonids.
Infectious pancreatic necrosis virus (IPNV) was isolated and
identified from asymptomatic adult coho (Oncorhynchus kisutch) and
chinook (0. tshawytscha) salmon returning to the Columbia River
system. Infectious pancreatic necrosis virus...
This study was undertaken to compile a comprehensive
review of the literature and to gain a better understanding
of the host-parasite relationship in corynebacterial kidney
disease of salmonid fishes. An indirect fluorescent antibody
technique was developed which allowed differentiation
of intra- and extracellular bacteria. This technique was
used in combination...
Renibacterium salmoninarum produces bacterial kidney
disease (BKD) in salmonid fish. Soluble antigen(s) (SA) produced
by the bacterium was characterized by physicochemical,
functional and immunological methods. The molecular weight
determination by SDS-PAGE analysis of SA yielded 14 different
molecular weight species. Seven of these species were
prominent, 57 kd protein being...
Selected secreted and cellular virulence factors of Aeromonas
salmonicida were examined. A protocol was developed for the
separation of two secreted proteases (P1 and P2 protease), and
a trout erythrocyte specific hemolysin (T-lysin) from
supernatants of cultures of the bacterium. Distinctions between
the proteases were demonstrated using molecular weight
determinations,...
Little is known about the virulence factors of Renibacterium
salmoninarum, the causative agent of salmonid bacterial kidney disease.
The predominant protein produced by R. salmoninarum in broth culture or
during infection is a 57/58 kDa protein (p57) which is associated with strain
virulence. In this study monoclonal antibodies (MAbs) to...
Detection and treatment of bacterial kidney disease (BKD)
was investigated. Experiments were conducted to evaluate the
quantitative, fluorescent antibody technique (QFAT) that is used
to detect, identify, and quantify both typical and 'bar form'
Renibacterium salmoninarum cells. Smears of kidney tissue from
naturally and artificially infected salmonids, both with and...
Morphological, cultural, biochemical and serological characteristics of
33 bacterial strains, thought to be similar to Cytophaga psychrophila, the
causative agent of bacterial cold-water disease were compared. Bacterial
strains identified as C. psychrophila were obtained from diseased salmonids
collected at widely separated geographic locations, and from fish with
pathological signs different...