Honey bees (Apis mellifera) are responsible for approximately $17 billion in crop production per year in the United States, and are arguably the most important pollinators in the nation. The future of crop pollination and production is threatened by widespread national honey bee colony losses, which have averaged approximately 30%...
Escherichia coli double-strand uracil-DNA glycosylase (Dug) was purified to apparent homogeneity from bacteria that were defective in uracil-DNA glycosylase (Ung). After cloning the dug gene, recombinant Dug was overexpressed, purified, and characterized with respect to activity, substrate specificity, product DNA binding, and mechanism of action. Purified Dug excised both uracil...