Deep sequencing analysis of an asymptomatic grapevine revealed a virome containing five RNA viruses and a viroid. Of these, Grapevine leafroll-associated virus 7 (GLRaV-7), an unassigned closterovirus, was by far the most prominently represented sequence in the analysis. Graft-inoculation of the infection to another grape variety confirmed the lack of...
The advent of improved DNA sequencing technologies has allowed the analysis of various microbial communities. Bloom-forming freshwater cyanobacteria can produce toxins and taste-and-odor compounds that can negatively affect drinking water supplies. Here, I have employed second- and third-generation sequencing technologies to characterize bloom-forming freshwater cyanobacterial genomes and their associated heterotrophic...
Viruses are obligatory parasites that depend on host cellular factors for their replication as well as for their local and systemic
movement to establish infection. Although myosin motors are thought to contribute to plant virus infection, their exact roles
in the specific infection steps have not been addressed. Here we...
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of Tobacco Mosaic Virus
Amari, K., Di Donato, M., Dolja, V. V., Heinlein, M. (2014) Myosins VIII and
Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if...
Virus-infected plants accumulate abundant, 21–24 nucleotide viral siRNAs which are generated by the evolutionary
conserved RNA interference (RNAi) machinery that regulates gene expression and defends against invasive nucleic acids.
Here we show that, similar to RNA viruses, the entire genome sequences of DNA viruses are densely covered with siRNAs in...
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,
Kristin Kasschau3, Valerian V. Dolja3, Patricia Otten4, Laurent Farinelli4, Mikhail M. Pooggin1*
1
Background:
The Amazon River runs nearly 6500 km across the South American continent before emptying into the western tropical North Atlantic Ocean. In terms of both volume and watershed area, it is the world’s largest riverine system, affecting elemental cycling on a global scale.
Results:
A quantitative inventory of genes...
Agrobacterium tumefaciens is a plant pathogenic bacteria which incites
crown gall tumors. Although virulence has been associated with a
97-156 Mdalton "tumor-inducing" (Ti) plasmid, few potential plasmid gene
products have been identified. The objective of this study was to
identify plasmid-coded gene products through a comparison of protein
fingerprints of...
Pallidosis is a disease of strawberry identified in 1957 with an unknown etiology. Two previously uncharacterized viruses in strawberry, Strawberry pallidosis associated virus (SPaV) and Beet pseudo-yellows virus (BPYV), have been found associated with disease symptoms. The complete nucleotide sequence of both viruses was determined and molecular detection protocols developed....
Transcriptomics and gene expression profiling enables the elucidation of the genetic response of an organism to various environmental cues. Transcriptomics enables the deciphering of differences between two closely related organisms to the same environment and in contrast, enables the elucidation of genetic responses of the same organism to different environmental...