Microsatellites or simple sequence repeats (SSRs) are highthroughput sequence based DNA markers that have been exploited as tools for measuring genetic distance and diversity in evolutionary studies, protection of intellectual property, genetic resource conservation management, cultivar identification and molecular breeding of crop plants. We isolated and sequenced 1095 clones from...
Two labelled arginines, DL-[2,3,3,-²H₃]arginine, 8,
and DL-[ ¹³C, 2-¹⁵N]arginine, 9, were synthesized. These
were fed to cultures of Streptomyces griseochromogenes in
order to probe the mechanism of the apparent arginine-2,3-
aminomutase reaction occurring in biosynthesis of blasticidin
S, 1. In previous studies of the enzymes lysine-2,3-
aminomutase and tyrosine-2,3-aminomutase different...
Medium chain fatty acids (MCFAs) are important industrial feedstocks for
soaps, detergents and surfactants. Interspecific lines derived from Cuphea lanceolata
and Cup hea viscosissima are being domesticated as a temperate source of MCFAs.
These species also serve as model organisms for understanding the biosynthesis of
MCFAs in seeds. Various markers...
Early studies on the biosynthesis of Sarubicin A, 1, had suggested the
intermediacy of the novel aromatic amino acid 6-hydroxyanthranilic acid,
17. To investigate the biosynthetic steps beyond 17, [¹³C-¹⁵N carboxamide]
6-hydroxyanthranilamide, 27a, was synthesized, and fed to fermentations
of Streptomyces helicus. Incorporation of 27a into 1 provided suggestive
evidence...
Making quality and winterhardiness in barley are "ultimate" phenotypes composed of
component, quantitatively inherited traits. A 69-point genome map of the seven chromosomes of
barley was used, in conjunction with multi-environment phenotypes for grain yield and malting
quality, to determine the chromosome locations of quantitative trait loci (QTLs). A combined...