Oxidative phosphorylation and related reactions, particularly as
affected by 2, 4-dinitrophenol (DNP), were studied with mitochondria
and submitochondrial particles isolated from the flight muscle of the
blowfly (Phormia regina) and housefly (Musca domestida). In the
presence of a phosphate acceptor, the mitochondria oxidized pyruvate
rapidly, and this was tightly coupled...
The consistent application of phosphatase inhibitors and a novel final purification step using a connected series of DE-51, DE-52, and DE-53 anion exchange chromatography columns facilitate the preparation of electrophoretically homogeneous sub-pupulations of rabbit muscle phosphofructokinase which differ in their catalytic properties and endogenous covalent phosphate contents. A band of...
An intermediate of oxidative phosphorylation was apparently
solubilized from cabbage mitochondria. The method of solubilization
used primarily was extraction of a mitochondrial acetone powder
with 0.1 M glycylglycine, pH 7.4, at 4° C. The intermediate
betrayed its presence by causing the transfer of Pi³² to ADP with
the resultant formation...
The interaction of skeletal muscle phosphofructokinase
with a variety of acidic proteins including calmodulin and
troponin C and with nucleic acids (RNA and DNA) is manifest
in a reversible, time-dependent loss of catalytic activity.
The inactivation is affected by a number of factors.
Substrates (fructose-6-phosphate and ATP), positive
effectors (fructose-2,6-bisphosphate,...
This thesis examines the interconnection between the in vitro and in
vivo phosphorylation of rabbit muscle phosphofructokinase. The first goal
of the project was to show whether a novel site of rabbit muscle
phosphofructokinase that is subject to in vitro phosphorylation, serine 376,
may also become phosphorylated in vivo. Evidence...