Despite the advances in surgery, physical therapy, and pharmaceutical agents, muscle
dysfunction (i.e., atrophy and weakness) continues to impair recovery from an anterior
cruciate ligament (ACL) injury and surgery. Ischemia-reperfusion injury during surgery
and the subsequent limb disuse are two events experienced by patients having ACL
surgery. Oxidative stress and...
This study was conducted to assess whether healthy adults compared with those with Metabolic Syndrome (MetS) have different mean oxidative-reductive potentials. Static oxidation-reduction potentials and capacity oxidation reduction potentials (sORP and cORP, respectively) were measured in plasma samples. The RedoxSYS® measures sORP in millivolts (mV), which is proportional to the...
Cognitive impairment, or cognitive decline, a noticeable and measurable decline in cognitive abilities (e.g. memory and learning) that exceeds those attributed to normal aging, represents an early symptom of neurodegeneration and increased risk for progression to more severe dementias, such as Alzheimer's disease (AD). While the complex etiology of these...
Vitamin E (VitE) is necessary for vertebrate embryonic development. VitE prevents lipid peroxidation (LPO), which requires detoxification by cellular antioxidant systems subsequently involving reducing power derived from energy metabolism. Thus, VitE protects metabolic networks in the developing embryo and the integrated gene expression networks compensating for and impaired by LPO-induced...
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_____________________________________________________________________
MaretG. Traber
Vitamin E (VitE) is necessary for vertebrate embryonic development. VitE
The primary goal of my study was to evaluate the effects of high dietary vitamin E on the rodent skeleton. The outcomes from my study assess the impact of high intakes of vitamin E and increased concentrations of its metabolite, carboxy ethyl hydroxy chromanol (CEHC), on bone turnover and skeletal...
The primary goal of my studies was to elucidate the mechanisms for the well-recognized interaction between two nutrients, vitamins E and K. The outcomes from my studies assess mechanisms for adverse effects of vitamin E and provide novel information on mechanisms for vitamin K homeostasis. These findings will provide information...
The present study was undertaken to analyze the vitamin E, total lipids, and cholesterol in regular and fat-reduced milk to assess whether decreases in fat result in lower vitamin E contents. Milk samples of varying fat contents (11%, 3.3%, 2%, 1% and 0.5% fat) were obtained from a local dairy...
In rats, sesame lignans increase plasma γ-tocopherol concentrations and inhibit γ-tocopherol degradation to its metabolite γ-carboxyethylhydroxychroman (γ-CEHC). To test if sesame lignan consumption inhibits γ- tocopherol metabolism in humans, muffins prepared with either corn oil (control) or sesame oil and an equimolar mixture of deuterium labeled d6-α- and d2-γ- tocopheryl...
During extreme exercise, athletes experience increased inflammation that is similar to the acute phase response. Endurance athletes, distance runners in particular, are also more susceptible to compromised iron stores. This study evaluated inflammation, immune function and iron status in athletes completing a 50K ultramarathon. Twenty-two well-trained distance runners, 11 males...
Patients with end-stage renal disease undergoing hemodialysis have a high incidence of oxidative stress-related diseases. This study evaluated oxidative stress and inflammatory markers in patients undergoing hemodialysis before and during vitamin E supplementation. Blood samples were obtained before and after dialysis during two separate dialysis sessions to establish baseline measurements....
Vitamin E (vitE) is a group of lipid-soluble antioxidants- of which alpha-tocopherol is the most biologically active and only form considered to be an essential nutrient. Through preferential binding and trafficking, the alpha-tocopherol transfer protein (α-TTP) regulates the concentration of vitE in the body. The impact of vitE deficiency on...
The relative enrichment of skin sebaceous gland lipids with deuterium-labeled α-tocopherol was compared with plasma enrichment to evaluate the delivery of vitamin E to skin. For the first week of this study, each subject consumed a daily dose of deuterated vitamin E (150 mg of an equimolar mixture of RRR-α-[5-(C²H₃)]-(d₃)...
The present study investigated whether vitamin E supplementation reduced oxidative stress in erythrocytes and improved vitamin E status in patients undergoing hemodialysis (HD). Plasma and erythrocyte α-tocopherol, plasma ascorbic acid, and iron status were determined in 11 regular HD patients prior to and post-dialysis, before and during oral supplementation of...
To determine if 6 weeks prior supplementation with vitamins E and C
could alleviate exercise-induced DNA damage, prevent lipid peroxidation and
inflammation, slow the rate of vitamin E utilization and/or attenuate muscle
damage, 22 subjects (11 females: 11 males) were studied during a 50 km (32
mile) ultramarathon. Subjects were...
Arachidonic acid (ARA) and docosahexaenoic acid (DHA) are polyunsaturated fatty acids required for proper embryonic development, specifically neurodevelopment. However, little is known regarding their conversion to other metabolites during embryogenesis. The oxidation of ARA gives rise to the biologically active eicosanoids and the oxidation of DHA gives rise to the...
To determine whether extreme endurance exercise induces lipid peroxidation, we studied 14 athletes (5 females: 9 males) during a 50 km ultramarathon (trial 1) and during a sedentary protocol (trial 2) one month later. At dinner the evening before the race (or sedentary trial), subjects consumed vitamin E labeled with...
Vitamin E was first described in 1922 as an unknown factor required for impregnated rats to carry their offspring to term. In fact, when vitamin E was chemically characterized it was given the name "tocopherol" derived from the Greek: tokos = childbirth; phero = to bear; and –ol, indicating an...
Polycyclic aromatic hydrocarbons (PAHs), including benzo[a]pyrene (B[a]P), are environmental pollutants linked to increased disease susceptibilities. Alpha-Tocopherol (αT) supplementation decreases B[a]P-DNA adducts in smokers, particularly women; but the mechanism is unknown. To test the hypothesis that αT protection from B[a]P exposure is gender-dependent, male and female rats received 7 daily subcutaneous...
Neurofibromatosis type 2 (NF2) is a genetic disorder caused by the inactivation of the
merlin tumor suppressor gene. NF2 patients develop bilateral vestibular schwannomas
(VS) and other nervous system tumors with no effective drug treatment option. In
pathological conditions, including NF2, production of the oxidant peroxynitrite leads to
protein tyrosine...
Neurofibromatosis Type 2 (NF2) is a genetic tumor disorder caused by mutations that inactivate the gene coding for the Merlin tumor suppressor. Patients with NF2 develop multiple tumors throughout the nervous system, mainly schwannomas, for which there is no cure or effective treatment. In NF2, redox signaling and oxidative stress...
Linear oligosacchardies of 1,4 linked β-D-glucopyranose are commonly referred to as cellodextrins (CD) or cellooligosaccharides (CO). They are of interest to those working in disciplines involving cellulose chemistry because they are often used as model substrates for cellulose itself. They are of interest to food scientists and nutritionists because they...
Milk fever (MF), retained placenta (RP), and left displaced abomasum (LDA) are three common and costly metabolic diseases in cows during the first days of lactation. Some studies suggest that circulating concentrations of α-tocopherol (ATOC) are decreased by these three diseases. It is, however, unknown if and how long lower...
In the presence of internal or external stressors, the body requires sources of energy that aide cells to combat inflammation. Fatty acids are an important source of energy and are vital components of cell membranes. Dietary fatty acids (n-6 and n-3) are of importance in immune function because they are...
The goal of this dissertation was to improve our abilities in acquiring critical in vivo data by establishing embryo-larva zebrafish as an exemplary model to quantitatively evaluate cancer progression. The living environment that encompasses a tumor has a significant effect on how cancer develops, grows and metastasizes. In order to...
Gadoid fish, like Alaska pollock and Pacific whiting, have a high level of trimethylamine-N-oxide demethylase (TMAOase) that catalyzes the breakdown of trimethylamine-N-oxide (TMAO) to formaldehyde (FA) and dimethylamine (DMA) even during frozen storage. FA reacts with amino acid residues to promote covalent cross-links in the formation of inter- and intramolecular...
Current dietary recommendations have placed increasing emphasis on dietary fat
as an important element to decrease risk of cardiovascular disease (CVD). Although total
fat and the fatty acid composition of diets influence the risk of CVD, the optimal amounts
of different fatty acids are not well defined, especially if n-6...
The thermal denaturation of proteins has been extensively studied using several methods including differential scanning calorimetry (DSC). A custom-built optical system was used to study thermal effects on protein as an alternative method to DSC measurements. It was used to investigate the thermal stability of bovine serum albumin (BSA) with...
Type 2 diabetes mellitus (T2DM) is a disease of multi-complications
affecting more than 20 million US adults. Hyperglycemia is the classic clinical feature of diabetes, and uncontrolled hyperglycemia leads to deadly health complications. Thus, control of blood glucose represents a major goal for diabetics. Human and rodent studies revealed another...
Doxorubicin is a widely used cancer therapeutic, but its effectiveness is limited by cardiotoxic side effects. Evidence suggests cardiotoxicity is due not to doxorubicin, but rather its metabolite, doxorubicinol. Identification of the enzymes responsible for doxorubicinol formation is important in developing strategies to prevent cardiotoxicity. In this study, the contributions...
BACKGROUND: Little is known about α-tocopherol’s bioavailability
as a constituent of food or its dependence on a subject’s age.
OBJECTIVE: To evaluate the α-tocopherol bioavailability from food,
we used collard greens grown in deuterated water (²H collard
greens) as a source of deuterium-labeled (²H) α-tocopherol consumed
by younger and older...
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without...
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, Lisa Truong d,e,f,
Robert L. Tanguay d,e,f, MaretG. Traber a,b,f,n
a Linus Pauling Institute, Oregon
Background: Nonalcoholic steatohepatitis (NASH) is a progressive form of nonalcoholic fatty liver disease and a risk
factor for cirrhosis, hepatocellular carcinoma and liver failure. Previously, we reported that dietary docosahexaenoic
acid (DHA, 22:6,n-3) was more effective than eicosapentaenoic acid (EPA, 20:5,n-3) at reversing western diet (WD)
induced NASH in LDLR-/-...
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Steatohepatitis in LDLR
[superscript -/-] Mice
Depner CM, Traber MG, Bobe G, Kensicki E, Bohren KM, et al. (2013
Background: Nonalcoholic steatohepatitis (NASH) is a progressive form of nonalcoholic fatty liver disease and a risk
factor for cirrhosis, hepatocellular carcinoma and liver failure. Previously, we reported that dietary docosahexaenoic
acid (DHA, 22:6,n-3) was more effective than eicosapentaenoic acid (EPA, 20:5,n-3) at reversing western diet (WD)
induced NASH in LDLR-/-...
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without...
We hypothesized that vitamin E (α-tocopherol) is required by the developing embryonic brain to prevent depletion of highly polyunsaturated fatty acids, especially docosahexaenoic acid (DHA, 22:6), the loss of which we predicted would underlie abnormal morphological and behavioral outcomes. Therefore, we fed adult 5D zebrafish (Danio rerio) defined diets without...
Background: Nonalcoholic steatohepatitis (NASH) is a progressive form of nonalcoholic fatty liver disease and a risk
factor for cirrhosis, hepatocellular carcinoma and liver failure. Previously, we reported that dietary docosahexaenoic
acid (DHA, 22:6,n-3) was more effective than eicosapentaenoic acid (EPA, 20:5,n-3) at reversing western diet (WD)
induced NASH in LDLR-/-...
Purpose:
The xanthophyll carotenoids lutein and zeaxanthin are concentrated in the macula lutea of the retina. It is thought that the two plant pigments may play a role in preventing the development of age-related macular degeneration (AMD). Exposure to damaging light plays a role in retinal deterioration and AMD. The...
Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
Zinc is an essential mineral that is integral to many proteins and transcription factors that regulate key cellular functions. The normal prostate accumulates high concentrations of zinc; however, malignant tissues have significantly lower zinc levels. This unique relationship between zinc and the prostate has sparked interest in the role of...
Non-alcoholic fatty liver disease (NAFLD) is defined as excess hepatic lipid accumulation, in the absence of excess alcohol consumption and chronic liver disease. NAFLD can range in severity from simple fatty liver (steatosis) to non-alcoholic steatohepatitis (NASH). NASH is defined as hepatic steatosis with inflammation and hepatic injury and describes...
The milk fat globule membrane, a major component in buttermilk, contains many complex lipids known to be involved in a variety of biological processes. Phospholipids, including sphingolipids, exhibit antioxidative, anticarcinogenic, and antiatherogenic properties and have essential roles in numerous cell functions. Filtration coupled with supercritical fluid extraction (SFE) may provide...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...
Dietary nitrate (NO3-), consumed primarily from leafy green and root vegetables, lowers blood pressure, improves vascular function and aerobic exercise performance. This performance-enhancing effect of nitrate supplementation is largely attributed to the increased bioavailability of the signaling molecule, nitric oxide (NO). Nitric oxide is a ubiquitous free radical signaling molecule...
Phytochemicals from cruciferous vegetables, such as broccoli, contain a multitude of bioactive compounds that prevent and suppress cancer and promote optimal health. Some of the health promoting effects of cruciferous vegetables are attributed to a class of compounds known as isothiocyanates (ITCs), a potent nuclear factor erythroid 2–related factor 2...
Approximately 12% of Americans do not consume the amount of zinc equal to the Estimated Average Requirement for zinc and could be at risk for marginal zinc deficiency. Zinc is an essential component of proteins in the defense against oxidative stress and DNA damage repair. Specifically in the prostate, zinc...
Metabolomics is a comprehensive analysis of small molecules, or metabolites, in a system. Metabolomics is a hypothesis-generating experiment and offers an unbiased analysis of cell metabolism that can aid in the understanding of fundamental biological processes. Metabolomics is widely and broadly applicable in the biological sciences and has been used...
Overtly non-toxic doses of organochlorine (OC) insecticides perturb lipid homeostasis
in mice and rats. To increase understanding of how chlordecone (CD), an OC
insecticide, modulates cholesterol (CH) homeostasis, we determined the effect of CD
pretreatment on the tissue and hepatic subcellular distribution of exogenous [¹⁴C]CH. Corn oil alone or CD...
Atherosclerosis is the underlying cause of ischemic heart disease and stroke and is the leading cause of death worldwide, especially in developed countries. As an inflammatory disease of arteries in a hyperlipidemic milieu, expression of adhesion molecules, such as endothelial-leukocyte adhesion molecule-1 (E-selectin) and intercellular adhesion molecule-1 (ICAM-1), on endothelial...
The consumption of cruciferous vegetables is associated with several health benefits, including cancer prevention. Many of these benefits are attributed to the phytochemical, sulforaphane (SFN), which is derived from cruciferous vegetables such as broccoli and broccoli sprouts. These vegetables contain glucoraphanin (GFN), SFN’s precursor, which is converted to SFN by...
Alcohol is a widely consumed, nonessential, bioactive nutrient with end-organ effects on the skeleton. Moderate levels of alcohol consumption are generally associated with increased bone mineral density (BMD), whereas higher intake levels result in reduced bone formation, bone loss, and increased risk for osteoporotic fracture. Given the negative impact of...
Prostate cancer risk is significantly lower in Asian countries compared to the US, which has prompted interest in the chemo-preventative action of dietary components such as soy and green tea commonly found in Asian diets, such as soy and green tea. Studies have suggested that soy isoflavones and green tea...
The following two projects have been carried out to clarify the impact of these foam stabilizers, and to compare their foam stabilizing power to one another as well as the cling ability. The first project investigated the impacts of four commercially available hop- derived foam stabilizers; iso-alpha-acid (Iso), rho(dyhydro)-iso-alpha-acid (Rho),...
Vitamin E improved liver histology in children and adults with NAFLD who participated in TONIC and PIVENS clinical trials,
but with significant inter-individual variability in its efficacy. Cytochrome P450 4F2 (CYP4F2) is the major enzyme
metabolizing Vit E, with two common genetic variants (V433M, rs2108622 and W12G, rs3093105) found to...
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, Rongrong Wei1, Min Zhang2, Shaochun Bai3, MaretG. Traber4,
Katherine Yates5, Oscar W. Cummings6, Jean
The hepatic α-tocopherol transfer protein (TTP) is required for optimal α-tocopherol bioavailability in humans; mutations in the human TTPA gene result in the heritable disorder ataxia with vitamin E deficiency (AVED, OMIM #277460). TTP is also expressed in mammalian uterine and placental cells and in the human embryonic yolk-sac, underscoring...
Vitamin E improved liver histology in children and adults with NAFLD who participated in TONIC and PIVENS clinical trials,
but with significant inter-individual variability in its efficacy. Cytochrome P450 4F2 (CYP4F2) is the major enzyme
metabolizing Vit E, with two common genetic variants (V433M, rs2108622 and W12G, rs3093105) found to...
Vitamin E improved liver histology in children and adults with NAFLD who participated in TONIC and PIVENS clinical trials, but with significant inter-individual variability in its efficacy. Cytochrome P450 4F2 (CYP4F2) is the major enzyme metabolizing Vit E, with two common genetic variants (V433M, rs2108622 and W12G, rs3093105) found to...
The temporal and spatial distribution of larvae of the cinnabar
moth (Tyria jacobaeae L.) correlates with changes in host plant qualiy.
An observed change in diet preference occurs in the 2nd instar
from low leaves to upper parts of generative plants. Timing of larval
attack and foraging pattern was found...
Prior to European settlement, native prairie dominated the landscape of the
Willamette Valley. Today, due to urbanization, agriculture, and the cessation of
burning, only isolated remnants of these grasslands still exist. In response to
conservation concerns, there has been a move to restore the remaining prairies in the
Willamette Valley,...
Infectious pancreatic necrosis (IPN) is an acute, contagious disease of trout fry
and fingerlings caused by viruses of the family birnaviridae. IPN disease usually results
in mortality that is inversely proportional to the age of the fish. While many studies
have been carried out to increase our understanding of IPN,...
Abdominal obesity is a part of insulin resistance syndrome that is closely
linked to increased risk of coronary artery disease (CAD). Because fat tissue acts as
an endocrine target and source of hormone production, increased metabolism or
production of chemical messengers in fat tissue may result in metabolic perturbations
that...
To test the hypothesis that embryogenesis depends upon α-tocopherol (E) to protect embryo polyunsaturated fatty acids (PUFAs) from lipid peroxidation, new methodologies were applied to measure α-tocopherol and fatty acids in extracts from saponified zebrafish embryos. A solid phase extraction method was developed to separate the analyte classes, using a...
Just as the structure of a protein determines its function, protein motions and lack-of-structure are also key to regulating protein behavior. The human eye lens is made up of high concentrations of extremely stable crystallin proteins, but even in this context, small motions over time can cause a loss of...
Background/Objectives: Advanced glycation end products (AGEs) are implicated in the pathogenesis of atherosclerosis, diabetes and kidney disease. The objective was to describe dietary intake, the dominant source of exposure to AGEs, with carboxymethyl-lysine (CML), a major AGE, in serum and urine, respectively.
Subjects/Methods: Serum and urinary CML were measured in...
Tocopherols are plant secondary metabolites that are known collectively as
vitamin E. Tocopherol compounds act as potent antioxidants by quenching free
radicals and protecting fatty acids from oxidative damage. In humans (in vivo)
tocopherols protect membrane lipids from breaking down, while in oils (in vitro)
tocopherols protect fatty acids from...
Egg fat is of considerable importance in the nutrition of the developing chick as a source of energy and essential fatty acids such as linoleic (18:2 n-6) and α-linolenic (18:3 n-3) acids. During incubation, yolk lipids provide fatty acids that are utilized for energy, synthesis of polyunsaturated fatty acids (PUFA)-rich...
Ascorbic acid, or vitamin C, is well known as a co-factor for proline-hydroxylase and as an anti-oxidant. However, it is also capable of forming covalent bonds, particularly in the role of a nucleophile, henceforth termed 'ascorbylation'.
The ascorbylation of electrophiles can occur under physiological conditions. Furthermore, there are a number...
Despite the advance of biomedical science, infectious diseases remain one of the number one killers in the world. They claim the lives of millions of people annually, particularly in underdeveloped and developing countries. In fact, more than 32% of the health burden in Africa is caused by infectious diseases, e.g.,...
This thesis documents a period of ecological and cultural change on a Willamette Valley, Oregon landscape. In particular, this study examines the Peavy Arboretum area and the cultural changes that accompanied the transformation of the landscape from an oak savannah in the mid-nineteenth century to a dense Douglas-fir forest in...
Alpha tocopherol plays a critical role in preventing lipid peroxidation within the body. Lipid peroxidation within the brain is hypothesized to be a possible mechanism of degenerative diseases such as Alzheimer’s and dementia. Due to the high amount of DHA, a long chain poly unsaturated fatty acid in the brain,...
Estuaries are once again emerging as important ecosystems for scientific study. Consequently, knowledge of what research has been conducted helps us identify benchmarks and plan new projects. A comprehensive bibliography of published research, technical reports, local documents, and data sets is one means of recording this knowledge. Yaquina Bay, located...
Climate change, wildfire, timber harvest, and land conversion alter the availability of downed wood in forests of the western United States. Numerous taxa rely on downed wood for temperature and humidity refugia, and downed wood may play a key role in enabling the persistence of climate-sensitive, low-vagility species like terrestrial...
This thesis is an IRB-exempt oral history focused on the non-profit Corvallis Multicultural Literacy Center (CMLC) in Corvallis, Oregon. The CMLC, formerly on 9th Street, was known to many community members as the Yellow House. The Yellow House was a dedicated community-based space where people of all cultures could come...
Explaining restoration failure can be as important as touting success. We used a series of studies to understand the failure of techniques commonly used to restore wetland prairies in the Willamette Valley of western Oregon. Burning, fallowing, and solarization (covering tilled plots with plastic sheeting to heat the soil) had...
Red raspberry, Rubus idaeus L., is a valuable crop for the U.S. Pacific Northwest and clonal propagation is required to produce disease-free plants and for germplasm preservation. One challenge of red raspberry micropropagation is the wide variation in growth response among the cultivars. The studies described in this dissertation were...
Clinical mastitis (CM), the most prevalent and costly disease in dairy cows, is diagnosed most commonly shortly after calving. Current indicators do not satisfactorily predict CM. This study aimed to develop a robust and comprehensive mass spectrometry-based metabolomic and lipidomic workflow using untargeted ultra-performance liquid chromatography high-resolution mass spectrometry for...
Oregon's estuaries are important ecosystems for scientific study. Consequently, knowledge of what research has been conducted helps us identify benchmarks and plan new projects. A comprehensive bibliography of published research, technical reports, local documents, and data sets is one means of recording this knowledge. For these reasons, the Guin Library...
This talk provides an overview of Linus Pauling's life and work, discusses the background and composition of the book "Visions of Linus Pauling," and touches on some themes in the history of the Linus Pauling Institute of Science and Medicine.
Increasingly, natural resource management agencies and nongovernmental organizations are sharing monitoring data across geographic and jurisdictional boundaries. Doing so improves their abilities to assess local-, regional-, and landscape-level environmental conditions, particularly status and trends, and to improve their ability to make short-and long-term management decisions. Status monitoring assesses the current...
The use of zebrafish in behavioral neuroscience is rapidly growing. Zebrafish can be assessed for alterations in multiple behavioral endpoints, creating opportunities to use this powerful model to identify chemicals that alter behavioral phenotypes. To evaluate the utility of zebrafish for neurotoxicity research, we designed custom instrumentation to evaluate numerous...
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. Andrea Knecht
Dr. MaretTraber
Melissa McDougall
Eric Johnson
Carrie Barton
Rest of Tanguay Lab
Funding
Two experiments were conducted to determine the effect of various levels of vitamin E on egg yolk. A diet of corn-flax-soybean meal was fed to layers and α-tocopherol content in eggs, egg production, and egg quality were evaluated. In experiment 2, vitamin E toxicity was also determined. In experiment 1,...
SCOPE: Aim of the study was to identify and monitor metabolite markers of dry bean consumption
in parallel human and mouse studies that each had shown chemopreventive effects of dry
bean consumption on colorectal neoplasia risk.
METHODS and RESULTS: Using LC/mass spectroscopy ± ESI and GC/mass spectroscopy, serum
metabolites of...
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] Lebold, K. M., Ang, A., Traber, M. G., Arab, L., Uri-
nary �-carboxyethyl hydroxychroman can be used as a
Purple tomatoes (Lycopersicon esculemtum) were bred by incorporating
genes that result in anthocyanin production in the skin of fruits. These genes
originally came from related wild species as no naturally occurring genes encoding
fruit anthocyanin expression are known within the cultivated species. By
combining Aft or Abg with atv or...
There were two main purposes to this study. The first was to determine the vitamin B-6 and pyridoxine β-glucoside content of selected foods commonly consumed in Malawi. The second was to examine the effect of preparation procedures of foods in Malawi on the content of vitamin B-6 and pyridoxine β-...
Well-functioning food webs are fundamental for sustaining rivers as ecosystems and maintaining associated aquatic and terrestrial communities. The current emphasis on restoring habitat structure-without explicitly considering food webs-has been less successful than hoped in terms of enhancing the status of targeted species and often overlooks important constraints on ecologically effective...
Native prairies, which once dominated the landscape of the Willamette Valley, are considered among the rarest of Oregon's ecosystems. Even though only remnants remain today, they harbor rare and endangered species such as the Fender's blue butterfly (Icaricia icariodes fenderi) and its preferred host plant, Kincaid's lupine (Lupinus sulphureus ssp....
Polycyclic aromatic hydrocarbons (PAHs), including benzo[a]pyrene (BP), are environmental pollutants linked to increased disease susceptibilities. Alpha-Tocopherol (aT) supplementation decreases BP-DNA adducts in smokers, particularly women; but the mechanism is unknown. To test the hypothesis that aT protection from BP exposure is gender-dependent, male and female rats received 7 daily subcutaneous...
Native upland prairies, which once dominated the landscape of the Willamette Valley, are considered among the rarest of Oregon's ecosystems. Even though only remnants remain today, they harbor many rare and endangered species such as the Fender's blue butterfly and its host plant, Kincaid’s lupine. Invasion of remnant prairies by...
Amyotrophic Lateral Sclerosis (ALS) is a neurodegenerative disease where paralysis of the body is the result of motor neuron function loss. A potential cause of ALS is the loss of zinc from Superoxide Dismutase (SOD). SOD’s cellular responsibility is to enzymatically react with the superoxide anion forming oxygen and the...
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Metallothionein and
note its ability to bind zinc (1961).
Maret and Vallee proposed a novel
cell functioning
The use of zebrafish in behavioral neuroscience is rapidly growing. Zebrafish can be assessed for alterations in multiple behavioral endpoints, creating opportunities to use this powerful model to identify chemicals that alter behavioral phenotypes. To evaluate the utility of zebrafish for neurotoxicity research, we designed custom instrumentation to evaluate numerous...
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Acknowledgements
Tanguay Lab
Dr. Robert Tanguay
Dr. Michael Simonich
Dr. Lisa Truong
Dr. Andrea Knecht
Dr. Maret
The purpose of this study was to examine the effect of extreme exercise on vitamin B-6
metabolism and urea nitrogen. Nine men and five women completed two 5-day trials; Trial 1
(T1) included a 50-km ultramarathon on day 4 and during Trial 2 (T2) subjects were "inactive"
on day 4....
Although assays have been done on the effects of vitamin E alpha-tocopherol (ɑ-tocopherol) on the lifespan of wildtype C. elegans, none have been done on the effects of the vitamin on mutant strains with mitochondrial mutations. By using a mev-1 genetic mutant strain of C. elegans with higher levels of...
Legumes are defined as the immature, germinated, or mature edible seeds of legume plants (Fabaceae). The primarily consumed legumes are dry beans, dry peas, lentils, chickpeas, cowpeas, fava beans, and pigeon peas, which are grouped as mature legumes, green beans and peas, which are considered green legumes, and sprouted or...
Strawberries {Fragaria ananasa) and raspberries {Rubus ideaus) are good sources ofmany important nutrients, and have great potential to improve human health. However, they are highly perishable and have a very short shelf-life mainly due to the attack of fungi and high respiration rate. Edible coatings, especially chitosan-based coatings have been...
The use of zebrafish in behavioral neuroscience is rapidly growing. Zebrafish can be assessed for alterations in multiple behavioral endpoints, creating opportunities to use this powerful model to identify chemicals that alter behavioral phenotypes. To evaluate the utility of zebrafish for neurotoxicity research, we designed custom instrumentation to evaluate numerous...
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the impact of micronutrients on embryonic development and function. The Traber
and Tanguay groups
Wine taste and mouthfeel are essential factors that determine consumer acceptance, consumer preference, and wine quality. The three taste descriptors, sweet, sour, and bitter, are well understood and routinely appearing in wine sensory. In contrast, information related to mouthfeel sensations is still relatively limited. Winemakers have worked hard to craft...
Signal transduction within and between cells is at the core of biological activity in all living systems. Signaling networks are required for regulating biological functions, including growth, development and survival. Deregulation of signaling cascades has been linked to chronic and acute diseases and disorders This thesis focuses on mass spectrometry...
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all
aspects of this dissertation.
Chapter 3. Dr. Robert L. Tanguay and Dr. MaretG. Traber