Injection of a 2.0 mg Cd
²⁺
/kg B.W. (I.P. as CdC1
₂
in
.05 M NaAc, pH = 6.9-7.2, .15 M or p = .15 with NaCl) resulted
in a decrease in ceruloplasmin (EC 1.12.3) p-phenylenediamine
oxidase activity within one hour of injection.
Maximal decrease (40%) occurred within six...
Selenium deficiency in rats results in increased carbon metabolism,
approaching twice the rate of selenium supplemented animals
(normal). This is also reflected in a 50 percent increase in lysosomal
enzyme activity in tissues of selenium deficient animals.
Isolation of rat liver lysosomes shows a 17 percent increase in
membrane thickness...
An in vitro study was conducted to investigate the metabolism and distribution of Se⁷⁵-selenite and Se⁷⁵-selenomethionine (SEM) in
chick blood. Se⁷⁵-selenite is taken up by RBC (13% within 20
minutes) and expelled into the plasma to become bound to proteins. In
contrast, Se⁷⁵-SEM showed a more gradual and continuous buildup...
Studies were conducted to investigate the metabolism of
selenium (Se) , and the influences of Se on the metabolism of cadmium (Cd) and inorganic mercury (Hg) in rats, and in ram semen in
vitro. Se-deficient (-Se) or Se-adequate (+Se) rats were injected intraperitoneally with either ¹⁰⁹CdCl₂ (5 μCi/rat) or ²⁰³HgN0₃...
The objective of our study was to investigate the effect of selenium on
selenoprotein W. Western blot analysis indicated that selenoprotein W is present in
muscle, brain, testis and spleen of rat tissues. Tissue distribution of selenoprotein W
was not altered in rats fed various selenium levels. Among muscle, brain,...
The objective of this study was to further determine the distribution of
selenoprotein W (SeW) in tissues from rats and sheep fed different selenium levels and to
search for the possible functions of this protein. In the rat study a total of 28 rat tissues
were examined and SeW was...
Rat selenoprotein W (SeW) promoter activity was investigated using different concentrations of cadmium, copper, and zinc. Two fragments (404bp and 1265bp) of the SeW promoter, containing a single metal response element (MRE), were ligated into the multiple cloning site of a pGL3-Basic reporter plasmid. The constructs were transfected into cultured...
Selenoprotein W (SeW) is a protein whose function is unknown, but potentially plays a vital role in calcium metabolism, as an indirect link has been established with white muscle disease (WMD). White muscle disease occurs in selenium deficient animals, and is characterized by the precipitation of calcium in muscle, leading...
Bioavailability of selenium (Se) in processed tuna and
wheat products was studied in humans and rats. The protein
source of the rat diets was torula yeast with Se supplied by
either raw, precooked or canned tuna, or whole wheat flour,
bread or bran. Sodium selenite was used as a control....