The design and construction of a chemiluminescence
photometer is described. The design is based on a discrete
sample system which has a number of advantages over
the previously described flow cell system and centrifugal
analyzer. The advantages include simple and inexpensive
equipment, small sample size, versatile electronic readout
system, and...
The application of lucigenin chemiluminescence for the determination
of trace concentrations of Co(II) is described. This method
is based on the enhancing effect that Co(II) has on the reaction of
lucigenin and hydrogen peroxide in a basic solution. The chemiluminescence
signal from a Co(II) enhanced lucigenin reaction, which is in...
A critical study of the cold vapor atomic absorption and fluorescence instrumentation for the analysis of mercury is described. Some of the important modifications involve the reduction vessel, absorption cell, fluorescence cell, and radiation source. The new modified systems have much greater calibration sensitivities and lower detection limits than those...
A multielement spectrometer has been developed for the simultaneous determination of four elements by atomic absorption (AA) or atomic fluorescence (AF). The spectrometer is based on a multiple exit slit monochromator and uses a mirrored funnel to direct light from the various exit slits to a single photomultiplier. There are...
The design, construction and operation of a fluorometric reaction
rate instrument is described. The instrument is designed for simple
operation with maximum versatility. Advantages and modes of
operation of the system are discussed in detail. The instrument is
used for the kinetics-based determination of trace levels of Ag and Al....
Improvements in instrumentation for fluorometric kinetic
measurements and the application of a new intensified diode array (IDA)
rapid scanning system to low level transient fluorescence measurements
are reported. This instrumentation is applied to a new fluorometric
kinetic method for thiamine (TM).
Modifications to the existing fluorometric reation rate instrument
include...
An on-line trace enrichment (TE) system, based on high pressure
liquid chromatogroaphy (HPLC) and flame atomic absorption (AA), has
been developed for trace metal analysis. The sample is pumped through
a column filled with a cation exchange resin which traps metal ions.
The ions are subsequently stripped from the column...
The development and optimization of a fluorometric reaction
rate method for the determination of aluminum is described.
Modifications to existing instrumentation permit automatic
monitoring and reporting of measurement conditions. Automatic
correction of measured analytical signals is provided if monitored
parameters deviate from optimum values. A computer controlled
standard addition procedure...
An automated instrument based on a single-board microcomputer
was developed for use in voltammetric analyses. Operator input of
numerous experiment parameters allows any type of voltammetric
analysis to be implemented and multiple scans to be run unattended.
A three operational amplifier potentiostat is used to control
the cell voltage and...
Studies of the use of NADH as the monitored fluorophore for enzymatic fluorescence kinetic methods are discussed. Interference studies
for the detection of NADH and the adaptation and optimization of fluorescence kinetic measurements to four serum assays are also described.
A microcomputer-controlled flow injection analysis (FIA) system was
designed and...
A sensitive technique for the determination of As(III) and As{V)
at μ/L and sub-μg/L concentrations from a single sample aliquot
has been developed. This technique is based on the hydride
generation atomic absorption (HGAA) spectrophotometry. The hydrides
of the arsenic species are generated in a reaction vessel by mixing
the...
An instrument was constructed to measure molecular fluorescence (FL) or chemiluminescence (CL) and absorbance (ABS) simultaneously. The lower, middle, and upper portions of the test solution are probed to obtain ABS, FL, and CL data. Optical fibers are used extensively. The ABS and FL or CL spectra are acquired in...
An automated multielement flame atomic fluorescence (AF) spectrometer based on microcomputer control was constructed to determine four elements simultaneously. The instrument employs a multiple exit slit monochromator where the light from various exit slits is directed to a single detector with a mirrored funnel. Each element is excited to fluoresce...
An automated radiochemical flow analysis (ARFA) system was developed which integrates chemical and radiochemical techniques into one system through the use of a microcomputer. The operator, through the microcomputer, controls valves and pumps to transfer sample and reagent solutions to counting loops, ion exchange columns and holdup reservoirs. A Ge(Li)...
An automated on-line ion exchange trace enrichment system has
been developed to improve the detection limits of flame atomic
absorption (AA) spectrophotometry in the determination of trace
metals in natural waters. The sample solution is passed through a
column of Chelex-100 chelating resin by a carrier buffer stream
delivered from...
This thesis is concerned with the development and use of routine
analysis techniques based on chemiluminescence (CL) detection for: 1)
ethanol in gin down to the 0.3% (v/v) level; 2) the antioxidants propyl
gallate and BHA in lard to levels as low as 0.3 and 0.2 mg/L, respectively; 3) pyrogallol...
The development and optimization of a single-phase derivatization
technique for small ring aromatics in water are presented. Also
described are the design, optimization and application of a remote
reaction chamber for fiber optic sensing.
Aqueous solutions of the major aromatic compounds in gasoline are
derivatized to o-nitrosophenol-Cu complexes with a...