Clinical methods to diagnose specific organ dysfunction have not been evaluated in fishes. To determine if an accepted clinical test of mammalian liver function could be applied to fishes, the
hepatic disposition of the organic anion sulfobromophthalein (BSP) was studied in rainbow trout (Salmo gairdneri) treated with a model hepatotoxicant....
Temperature influences the incidence of chemically induced cancer in fish,
with warmer temperatures being associated with higher cancer incidence. The
mechanisms of temperature-modulated chemical carcinogenesis in fish, however,
have not been described in detail. Therefore, one primary objective of this study
was increased understanding of how temperature-modulated genotoxicity of 7,12-...
Of 109 aquatic birnaviruses (AB) belonging to 9 serogroup A, all replicated and produced rapid and extensive cytopathology in CHSE-214 and RTG-2 cells, whereas only half produced significant levels of cytopathological effect (CPE) in two nonsalmonid cell lines tested (EPC and FHM). In many instances it was found that although...
The rainbow trout (Oncorhynchus mykiss) model
of chemical carcinogenesis is becoming increasingly
important as a supplement to rodent studies. However,
much of the molecular biology of the carcinogenic
response is still unknown in the trout model. The ras
gene family has been implicated in the tumorigenesis of
both spontaneous and...
The characteristics of an inhibitor of infectious pancreatic necrosis virus (IPNV)
found in normal rainbow trout serum (RTS) were studied. The serum inhibitor had a
molecular weight of approximately 150 kDa and was dependent on divalent cations,
either Ca²⁺ or Mg²⁺. It was stable at temperatures up to 50°C and...
For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
The acute phase response (APR) includes an early response of the innate immune
system to an inflammatory agent. Acute phase proteins (APP) are produced at
increased rates in the liver during this response. The APR has been well
characterized in mammals, but it is unclear to what degree teleosts mount...
The hypotheses to be tested in this investigation are: 1) there is a threshold level of liver lipid and glycogen content above which the health condition of hatchery-reared fish is impaired, 2) fatty and high glycogen livers impair liver function and structure, and 3) fatty and high glycogen livers increase...
Rainbow trout were exposed to aluminum at pH 7.25 and
8.25 and four hardnesses (10, 30, 80, and 120 ppm CaCO₃)
for 96 hours in a continuous-flow system and mortality and
aluminum accumulation in the gills were determined.
Temperature, pH, and dissolved oxygen were measured
daily for each treatment. Dissolved...
Chlorophyllin (CHL), a water-soluble derivative of the green plant pigment, chlorophyll, is an effective antimutagen and anticarcinogen in various model systems when used as a modulator against a class of carcinogens that, in general, have a structure consisting of at least three fused rings. Dibenzo[a,l]pyrene (DBP), an extremely potent environmental...
The virulence of selected isolants of infectious hematopoietic
necrosis virus (IHNV) were differentiated and correlated with
electrophoretic mobilities of virion structural proteins by sodium
dodecylsulfate polyacrylamide gel electrophoresis. A type 1
electropherotype of IHNV was more virulent for kokanee salmon
(Oncorhynchus nerka) and a type 2 strain more virulent for...
This dissertation describes two different projects. The first is the development of an oral DNA vaccine delivery system for fish. A novel oral DNA vaccine delivery system was developed for Rainbow Trout by combining non-viral vectors (polycationic liposomes or polycationic polymer) to facilitate the DNA vaccine's uptake by cell membranes...
A series of experiments with Aeromonas salmonicida and infectious
hematopoietic necrosis virus (IHNV) were carried out to determine dynamics of the
spread of infection in chinook salmon (1.2-1.98g) and rainbow trout (1.2-3.1g). It was
found in experiments with A. salmonicida that fish infected by bath immersion became
infectious at 4...
Over the last decade, the Oregon Department of Fish and Wildlife (ODFW) has documented a precipitous decline in the Crooked River redband trout (Oncorhynchus mykiss) population, prompting this study to address potential factors contributing to the decline. There are two main goals to this project: (1) identify potential factors contributing...
DNA fragments of the gene for VP2, the major capsid protein of Infectious
Pancreatic Necrosis Virus (IPNV), were expressed in Escherichia coli and
characterized immunologically with antisera made to different serotypes of
IPNV. The entire VP2 gene was excised from the plasmid pUC19/A+SΔK and
fragmented with restriction enzyme Sau3A1. The...