Transitional cell carcinoma (TCC), the most common cancer of the urinary bladder in dogs, is usually diagnosed at an advanced disease stage with limited response to chemotherapy. Commercial screening tests lack specificity and current diagnostic procedures are invasive. A proof of concept pilot project for analyzing the canine urinary proteome...
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Medlock
c
, Cheri 4
Goodall
c
, Samanthi Wickramasekara
d
, Claudia S. Maier
d
5
a
Department
Transitional cell carcinoma (TCC), the most common cancer of the urinary bladder in dogs, is usually diagnosed at an advanced disease stage with limited response to chemotherapy. Commercial screening tests lack specificity and current diagnostic procedures are invasive. A proof of concept pilot project for analyzing the canine urinary proteome...
Transitional cell carcinoma (TCC), the most common cancer of the urinary bladder in dogs, is usually diagnosed at an advanced disease stage with limited response to chemotherapy. Commercial screening tests lack specificity and current diagnostic procedures are invasive. A proof of concept pilot project for analyzing the canine urinary proteome...
The isothiocyanate sulforaphane is a promising molecule for development as a therapeutic agent for patients with metastatic
prostate cancer. Sulforaphane induces apoptosis in advanced prostate cancer cells, slows disease progression in vivo and is well
tolerated at pharmacological doses. However, the underlying mechanism(s) responsible for cancer suppression remain to be...
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-induced apoptotic
signaling in PC3 prostate cancer cells
Watson, G. W., Wickramasekara, S., Palomera
The isothiocyanate sulforaphane is a promising molecule for development as a therapeutic agent for patients with metastatic
prostate cancer. Sulforaphane induces apoptosis in advanced prostate cancer cells, slows disease progression in vivo and is well
tolerated at pharmacological doses. However, the underlying mechanism(s) responsible for cancer suppression remain to be...
The isothiocyanate sulforaphane is a promising molecule for development as a therapeutic agent for patients with metastatic
prostate cancer. Sulforaphane induces apoptosis in advanced prostate cancer cells, slows disease progression in vivo and is well
tolerated at pharmacological doses. However, the underlying mechanism(s) responsible for cancer suppression remain to be...
Background: Osteosarcoma (OSA) is the most common primary bone tumor of dogs and carries a poor prognosis
despite aggressive treatment. An improved understanding of the biology of OSA is critically needed to allow for
development of novel diagnostic, prognostic, and therapeutic tools. The surface-exposed proteome (SEP) of a
cancerous cell...
The Philippines’ labor export system is a complex web of policy instruments that have been amended, repealed, and refined over the last four decades. The laws along with the hortatory tools used to promote them have shaped the lives of millions of Filipinos, separating families across oceans. Temporary labor migration,...
State-of-the-art, high-resolution mass spectrometry techniques for acquisition of metabolomic and lipidomic data coupled with advanced computational methods provide new opportunities for interpreting large and complex datasets and comprehending the underlying biological processes of diseases. Both metabolomics and lipidomics strive to obtain comprehensive analyses of small molecules, i.e., metabolites and lipids,...
Alcoholic liver disease (ALD) continues to be one of the major public health problems in the United States and worldwide. Complicated by factors including gender, polymorphisms of alcohol-metabolizing enzymes, immunologic factors, exposures to other substances/drugs, hepatic viral infections, nutritional deficiencies, and obesity, ALD is a complex disease that requires a...
Proteins are essential to all biological systems. Proteins participate in numerous cellular processes by interacting with other proteins, other metabolites and membranes in a dynamic environment. Studying the structural and conformational properties of proteins in the solution phase is necessary to understand their protein folding and interaction dynamics. This research...
Metabolomics has recently gained momentum in biomolecule research and complements the genomics and proteomics research space. Metabolomics strives to detect, identify, and quantify all metabolites present in biological samples. In particular, biomolecular analysis using ultra-performance liquid chromatography combined with mass spectrometry (UPLC-MS) has become increasingly important for metabolomic analyses. Similarly,...
The analysis of oxidative stress-induced post-translational modifications remains challenging due to the chemical diversity of these modifications, the possibility of the presence of positional isomers and the low stoichiometry of the modified proteins present in a cell or tissue proteome. Alcoholic liver disease (ALD) is a multifactorial disease in which...
The post-translational modification of proteins by electrophilic oxylipids is emerging as an important mechanism that contributes to the complexity of proteomes. Enzymatic and non-enzymatic oxidation of biological lipids results in the formation of chemically diverse electrophilic carbonyl compounds, such as 2-alkenals and 4-hydroxy alkenals, epoxides and eicosanoids with reactive cyclopentenone...
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detection and identification
Yury V. Vasil’ev, Shin-Chen Tzeng, Lin Huang and Claudia S. Maier
Sulforaphane is an isothiocyanate derived from cruciferous vegetables and has been under investigation as a cancer chemopreventive agent for over two decades. The compound is well tolerated and has been shown to slow cancer progression in several different pre-clinical models of carcinogenesis, such as of the lung, breast and colon....
Oxidative stress is defined as an imbalance that favors the production of reactive oxygen species (ROS) over an organism's antioxidant defense. ROS have the ability to damage, either directly or indirectly, biomolecules including DNA, proteins, carbohydrates, and lipids. Various pathological conditions and environmental and chronic diseases have been associated with...
Obesity and metabolic syndrome (MetS) affect over 1.9 billion adults and 12.7 million youth in the US. If all of today’s obese American youth become obese adults, the total societal costs over their lifetime may exceed $1.1 trillion. We show that feeding rodents a high-fat diet supplemented with xanthohumol (XN)...
The Farnesoid X Receptor (FXR) is a member of the nuclear receptor superfamily of transcription factors that plays a key role in the regulation of bile acids, lipid and glucose metabolisms. The regulative function of FXR is governed by conformational changes of the ligand binding domain (LBD) upon ligand binding....