Pre-fermentation cold soak is a winemaking technique used to alter the color, mouthfeel, and aroma of red wine. While it is still debated what impact cold soak has on these wine quality parameters, current thoughts are that resident yeast populations found in fresh grape must play a role in altering...
Synthesis of total RNA, DNA, and protein was followed in a
haploid yeast auxotroph during starvation for required amino acids
and uracil, and during shift-up and shift-down conditions.
During amino acid starvation, synthesis of macromolecular
constituents was not immediately affected, reflecting the presence
of large intracellular amino acid pools. Under...
An enzyme system from cell-free yeast homogenates mediating the
transfer of the methyl group from S-adenosylmethionine-methyl-C¹⁴
or methionine-methyl-C¹⁴ to C-28 of ergosterol has been studied.
The effects of divalent cations, suithydryl inhibitors and reducing
substances on the activity of the enzyme system have been investigated.
It was established that for...
Two experimental approaches have been used to investigate the interaction of sterols with respiration in yeast. Cells were tested with an antifungal sterol, azasterol, and a mutant was obtained with an altered sterol composition. Polarographic measurements were done on mitochondria for both experiments. The effects of azasterol on mitochondrial respiration...
The structure and transcriptional activity of intranuclear
and isolated chromatin from logarithmically growing
yeast cells has been compared to chromatin from cells which
have entered the stationary phase and ceased growing. Both
chromatins show a similar nucleosomal repeat pattern and a
160 by repeat size when digested with staphlococcal nuclease....
The ability of cholestanol to support growth of yeast sterol
auxotrophs has been examined. Growth on this stanol was precluded
unless minute quantities of ergosterol were available, a phenomenon
designated sparking. The low levels of ergosterol were
insufficient to support growth alone. The data indicate that
cholestanol is fulfilling a...
Many different strains of yeast have been isolated from the natural
microflora on fruits. It has been known for some time that these
strains of yeast have distinct fermentation characteristics and, when
used in the production of wine, impart these characteristics to the
wine. These characteristics may be evidenced in...
An assay is described to measure sterol uptake in
Saccharomyces cerevisiae. This assay involves specific
binding of radioactively labelled sterol to actively
growing yeast cells.
It was found that uptake does not occur in stationary
cultures and that some growth is necessary for FY3 , a
sterol auxotroph, to take...
The positions of the DNase I hypersensitive sites within the
galactose gene cluster of the yeast Saccharomyces cerevisiaa have
been determined. When yeast nuclei were incubated with DNase I, the
purified DNA restriction endonuclease digested and analyzed by
Southern hybridization, five hypersensitive regions were observed in
this cluster of three...