This report describes the isolation of a new virus from adult
chum salmon (Oncorhynchus keta) returning to the Tokushibetsu Hatchery,
Hokkaido, Japan. The agent was isolated in the CHSE-214 cell line
derived from chinook salmon (Oncorhynchus tshawytscha). The virus
replicated in selected fish cell lines incubated between 10 and 20°C...
Chum salmon (Oncorhynchus keta) were artificially
infected with erythrocytic necrosis virus (ENV) to study
the physiological and hematolgical consequences of ENV-infection.
Infected and control fish were held in
pathogen-free seawater and sampled weekly for five weeks.
Physiological tests included plasma cortisol, glucose,
protein, and osmolality, blood lactic acid, and liver...
The primary objective of this study was to identify
and investigate the underlying basis for variation in the
volume of exports of Canadian chum salmon during 1954-1975
period. A number of factors thought to be of importance
in the determination of the volume of exports of chum
salmon were hypothesized....
The effects of temperature and ration on growth, food consumption, and food conversion efficiency of chum salmon, Oncorhynchus keta, in sea water were studied in two experiments conducted during the spring, summer, and fall, 1973, at the Oregon State University Marine Science Center, Newport, Oregon. The test temperatures
employed in...
Within the tested range of 1000 - 7000 eyed eggs/ft² gravel substrate (1.08 - 7.53 eggs/cm²), the optimum stocking density for chum salmon (Oncorhynchus keta) eggs in shallow matrix substrate incubators occurred at 3000 - 4000 eggs/ft². Premature fry, which predominated early emergence, showed greater variability in lipid content than...
Effects of the marine environment on age and size at maturity, early marine growth, and abundance of chum salmon, Oncorhynchus keta, were studied at Olsen Creek during 1959-77. Chum salmon returned to Olsen Creek as predominately 3-, 4-, and 5-year fish; however, age composition varied from year to year. The...
Chum salmon from different stocks were bred together in two experiments. Three stocks contributed gametes to one experiment, two stocks to another. Sibling groups of eggs, alevins, and fry were maintained in a common environment. Variability of development rates, rearing performance, susceptibility to disease, and behavioral traits was partitioned into...
Estuarine residence and growth of juvenile chum salmon
(Oncorhynchus keta) from Netarts Bay, Oregon were estimated
from daily-formed growth increments of sagittal otoliths
which are distinguishable from accretion patterns formed
during freshwater residence. Estuarine residence time was
inversely related to the average size at which juvenile chum
salmon entered Netarts...
"The chum salmon pilot production project at Netarts Day, Oregon, is intended to develop a technology for commercial application of extensive salmonid culture on the one hand, and to demonstrate that technology on the other. This report reviews operations attendant to the pilot production of chum salmon at Netarts Bay...
Ages were determined for 65 fish from the 1947 run and for 287 fish from the 1949 run. In 1947 the percentage composition by age was as follows: 32.3 percent 3-year-old fish, 66.2 percent 4-year-old fish, and 1.5 percent 5-year-old fish. An approximate 95 percent confidence interval for the true...
Tillamook Bay chum salmon are caught commercially by gill-nets, both set and drift, and from 1928 through 1949 the landings have averaged 819,689 pounds per season. More chum salmon are caught on Tillamook Bay than on the rest of the Oregon coastal rivers combined. These fish enter the ocean only...
Characterization of a virus requires that its physical, biochemical
and biological properties be determined. In this study the
Oregon sockeye salmon virus was characterized. It was isolated
in the fall of 1958 from juvenile sockeye salmon, Oncorhynchus
nerka, being reared at the Oregon Fish Commission's Willamette
River Hatchery.
When inoculated...
Samples, consisting of scales, length and weight measurements, and sex determinations of chum, pink, and silver salmons, were taken from the commercial catch in the Columbia River in 1914. Five hundred eighteen chum scales were examined. All fish had gone to sea early in their first year; and 70.5 percent...
The detection and antigenic nature of the causative Corynebacterium
of bacterial kidney disease and chemotherapy of this disease
in juvenile coho salmon (Oncorhynchus kisutch) were examined.
Each of 207 yearling coho salmon collected from a population
undergoing a severe epizootic of bacterial kidney disease were examined
for the presence of...
Since the isolation of the Oregon sockeye salmon virus (OSV) in
1958, extensive investigations have been undertaken to characterize
the properties of this virus. The results of these investigations
have indicated that OSV is a single-stranded RNA virus which contains
essential lipids and has a density of 1.16 gm/cm³ in...
An oral vaccine was developed for control of vibriosis
in Pacific salmon. The vaccine consists of a lyophilized
sonicate of cells of Vibrio anguillarum, the causative
agent of this disease. The preparation was incorporated
into a ration and fed to salmon in fresh water before the
fish were transported and...
Studies were directed toward the formulation of a selective
Aeromonas medium that would permit the isolation of Aeromonas
species in the presence of other common bacterial flora. The final
composition of this medium, PBG Agar, in grams per liter, is:
Bacto-peptone, 10; Bacto-beef extract, 10; glycogen, 4; NaCl, 5;
sodium...
The Oregon strain of the agent of infectious hematopoietic necrosis (IHN) was isolated from fingerling sockeye salmon (Oncorhynchus nerka) by J. L. Fryer in 1958. Before the study reported herein no other virus had been recovered from any fish population in Oregon. Therefore, in July, 1971 a survey was initiated...
Juvenile coho salmon (Oncorhynchus kisutch) were orally
immunized against the bacterium Flexibacter columnaris (formerly
Chondrococcus columnaris), the causative agent of columnaris disease
in fish, with a vaccine-food preparation containing formalin-killed
cells. Fish immunized for 1, 2, 3, and 4 months were challenged
with selected concentrations of F. columnaris by exposure...
Efficacious vaccines were developed for the control of Vibrio
anguillarum, the etiological agent of vibriosis in salmonid fish.
These bacterins can be administered either orally or parenterally.
It was determined that both formalin-killed lyophilized whole cells
and wet-packed whole cells of the organism are effective oral immunogens.
Intraperitoneal injection of...
Juvenile coho salmon (Oncorhynchus kisutch) of three transferrin
genotypes (AA, AC and CC) were experimentally infected with the
causative agent of bacterial kidney disease (BKD) and mortalities
observed. Six experimental and control groups were used:
(1) bacteria-infected + no Fe⁺³ ; (2) bacteria-infected + low Fe⁺³
levels; (3) bacteria-infected +...
The distribution in Oregon of two viruses affecting salmonid
fishes was determined by examination of hatchery and feral fish.
Infectious pancreatic necrosis virus (IPNV) was isolated from fish
at the Oregon Department of Fish and Wildlife Research Laboratory,
Corvallis, Roaring River, Fort Klamath, and Wizard Falls Hatcheries.
Fish collected at...
Experiments were designed to more precisely evaluate the effects of water temperature on the progress of bacterial kidney disease ( BKD) in three species of salmonid fishes. Infections were produced by intraperitoneal injection of suspensions of the causative organism into fish held at seven temperatures ranging from 3.9 to 20.5°C....
Fish diseases and various parameters associated with disease
caused mortality of fish were monitored at the Oregon State University
Marine Science Center and at a private mariculture facility on
Yaquina Bay during a period of five years. Nearly all disease problems
observed were caused by Vibrio anguillarum and Vibrio spp....
The relative resistance of the coho salmon (Oncorhynchus kisutch) and the chinook salmon (O. tshawyscha) to the parasitic larvae (glochidia) of the freshwater mussel (Margaritifera margaritifera) was studied in an attempt to determine why the coho appeared to be more resistant. Natural and acquired antibody was sought in the mucus...
Glochidial development in freshwater mussels (Mararitifera margaritifera) located in the Siletz River, Oregon, was completed in 13 days at an average water temperature of 12.8 C. Glochidia were released by these mussels for 33 days, May 13 to June 15, 1971. The comparative susceptibility of four species of salmonid
fishes,...
A nested mating experiment in which 20 males were each mated
to four different females was used to obtain an estimate of the heritability
of resistance to death from gas bubble disease in Columbia
River fall chinook salmon (Oncorhynchus tshawytscha). Heritability
estimates ranged from 0. 037 to 0. 038.
Bioassays...
The purpose of this study was to characterize Cytophaga
psychrophila isolates obtained from coho fingerlings with low temperature
disease collected at selected hatcheries in Oregon. In
addition, cultures thought to be related to this bacterium were
isolated from Siletz Hatchery water, deformed juvenile and spawning
adult coho salmon, rainbow and...
Experiments were conducted to determine the effects of feeding
sulfamethazine to chinook salmon (Oncorhyncus tshawytscha, Walbaum)
as it is used in the Oregon Moist Pellet diet (OMP) for control of
bacterial infections and to compare this drug with sulfisoxazole and
sulfadimethoxine. These drugs were compared in the following manner:
toxicity,...
This project was initiated to determine the incidence, distribution
and species of fish affected by Ceratomyxa shasta in Oregon
waters. Returning adult Pacific salmon and steelhead trout were
chosen for the survey because they were known to be susceptible to
infection. The parasite was also believed to be an important...
This thesis describes the viral susceptibility of two cell
cultures established from embryonic tissue of salmonid
fish, chinook salmon line CHSE-114 and steelhead trout line STE-137. The viruses used were Oregon sockeye salmon virus (OSV),
Sacramento River chinook disease virus (SRCD), Western equine
encephalitis virus (WEE) and Newcastle disease virus...
The purpose of this project was to study the possibility of controlling
furunculosis in salmonid fish using immunological procedures.
Antiserum with an agglutinating antibody titer of 1:20,480
against Aeromonas salmonicida was produced in a horse. This
hyperimmune serum was used to passively immunize coho salmon,
Oncorhynchus kitsutch, (jacks). However, this...
The Oregon sockeye salmon virus (OSV) was isolated from
diseased sockeye salmon (Oncorhynchus nerka) fingerlings in 1958
by J. L. Fryer. Experimentation performed prior to the research
reported herein indicated that the OSV contained essential lipids, was
100 to 300 mμ in size, and possessed RNA (presumptively identified
by 5-bromodeoxyuridine...
Unfertilized ova from spring chinook salmon (Oncorhynchus
tshawytscha) were examined for the presence of classical salmonid
inmmunoglobulin. Immunodiffusion techniques in which rabbit anti-salmonid
immunoglobulin was reacted against an ova homogenate
and rabbit anti-ova homogenate was reacted against immune chinook
serum failed to detect classical-type antibodies within these eggs.
A lectin...
Juvenile chinook salmon (Oncorhynchus tshawytscha) were experimentally infected with Renibacterium salmoninarum (Rs), the causative agent of bacterial kidney disease (BKD), to investigate the effects of BKD on three aspects of juvenile salmonid performance: (1) predator avoidance ability; (2) smoltification; and (3) physiological responses to stress. For these experiments, fish with...
The aquaculture industry in the UK has experienced substantial growth in
the past two decades. The productivity and competitiveness of the sector to
a great extent depends on the favourable health status that the sector enjoys
relative to other major salmon producing countries in Europe. Currently the
UK is subject...
An oral Vibrio vaccine for salmonids was developed.
The vaccine was produced by spray coating lyophilized
formalin-killed whole cells of Vibrio anguillarum (VA LS 1-
74) onto non-pareil sugar beads. Then methacrylic acrylic
acid copolymer (Eudragit L-30D) was applied as an enteric
protective coating.
Using x-ray radiographic techniques, it was...
This study¹ was undertaken: (1) to obtain information on the distribution of the snail, Oxytrema silicula, in three coastal rivers in Oregon, and the seasonal incidence of infection in these snails and in snails from an inland stream, with the cercariae of the trematode, Nanophyetus salinincola; (2) to follow cercarial...
The presence of the etiologic agent of "salmon poisoning"
disease, Neorickettsia helminthoeca, was demonstrated in eggs of
the trematode vector, Nanophyetus salmincola. Three dogs were
given 100,000 and one dog 82,000 ground fluke eggs by intraperitoneal
injection. The four animals developed "salmon poisoning"
disease and died. One of these dogs...
