Cryopreservation of pollen offers a simple and effective method of
long-term pollen storage. Pollen may be stored in liquid nitrogen (LN)
for many years without loss of its essential capabilities to pollinate,
fertilize, and set normal fruit and seed when used in plant breeding,
for controlled pollinations, or for the...
Effective methods for long-term preservation of cord red blood cells (RBCs) are needed to ensure a readily available supply of RBCs to treat fetal and neonatal anemia. Cryopreservation is a potential long-term storage strategy for maintaining the quality of cord RBCs for the use in intrauterine and neonatal transfusion. However,...
Cryopreservation nearly universally depends on the equilibration of cells and tissues with high concentrations of permeating chemicals known as cryoprotective agents, or CPAs. Despite their protective properties, CPAs can cause damage as a result of osmotically-driven cell volume changes, as well as chemical toxicity. In this study, we have used...
Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s ability to form a stable...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used...
Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to...
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
Cryopreservation of adherent cells may be advantageous for cell types that are difficult to
preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...
In the last decade, there has been renewed interest in the use of granulocyte transfusions to treat infections in individuals with compromised immune systems. However, granulocytes only remain functional for about a day after isolation and this short shelf life is a significant drawback. Cryopreservation would allow long term storage...
Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in
which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity.
Thus, we investigated the use of physics-based mathematical optimization to guide design of
cryoprotectant loading methods for mouse and human oocytes. We first...
Granulocytes are a class of white blood cells essential to immune function. Granulocyte transfusions hold promise to treat many conditions, but the short-shelf life of granulocytes makes transfusions infeasible without a cell preservation method. The purpose of this study was to investigate the potential for cryopreservation of granulocytes using 30%...
Successful cryopreservation of bone marrow in situ within vertebral bodies offers an opportunity for quick and convenient storage of a significant quantity of bone marrow stem cells, including hematopoietic- and mesenchymal-type stem cells. However, cryopreservation of more complex large tissues and organs, such as the vertebral body (VB), remains problematic....
BACKGROUND: Simple and effective cryopreservation of human oocytes would have
an enormous impact on the financial and ethical constraints of human assisted
reproduction. Recently, studies have demonstrated the potential for cryopreservation
in an ice-free glassy state by equilibrating oocytes with high concentrations of
cryoprotectants (CPAs) and rapidly cooling to liquid...
Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any future RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s ability to form a...
Successful cryopreservation of all biological specimens would have an untold positive impact on medicine and scientific research. However, cryopreservation of the most complex biological specimens, such as tissues and organs, remains elusive. Vitrification, or ice-free cryopreservation, is promising for cryopreservation of complex specimens. The biggest challenge, though, is the toxicity...
Alternating temperature (22°C / -1°C) pretreatments were effective for
increasing cold hardiness and recovery of cryopreserved meristems. Combining low
temperature pretreatment with abscisic acid (ABA) or sucrose treatments reduced the
time required for optimal cold hardiness of meristems. Desiccation tolerance was
directly correlated with increased cold hardiness and recovery of...
There is a significant need for technological advancement in blood processing. New technologies in rapid processing of frozen blood products could significantly improve management of the blood supply chain in the United States by enabling on-demand use of frozen blood products that can be stored for 10 years instead of...
Oxidative stress that occurs during cryopreservation may be reduced by the addition of antioxidants. Vitamin E (Vit E), Vitamin C (Vit C), glutathione (GSH), lipoic acid (LA), glycine betaine (GB) and polyvinylpyrrolidone (PVP) were applied at four steps of the PVS2 vitrification protocol (pretreatment, loading, rinsing, and regrowth). Shoot tips...
Even with advanced medical technology, there is still an unmet need for organs to reduce the transplant waiting list and to advance organ-related research. Cryopreservation through vitrification is one possible solution to increase organ availability. A major challenge with cryopreservation of organs is the toxicity of the cryoprotectants (CPAs) that...
Cultures of Streptococcus lactis and Streptococcus cremoris
were grown in sterile 11% nonfat milk (NFM) at 32 C for 17 hr with
the pH maintained at 6. 3 with sodium carbonate. The cells were
harvested and resuspended in a medium of 5% gelatin, 5% sodium
citrate, 2% monosodium glutamate and...
This study lays the groundwork on potential techniques that could be employed to improve the post-thaw wash processing of cryopreserved human red blood cells. Transfusion of red blood cells is one of the most commonly practiced procedures in clinical medicine. Millions of red blood cell units are transfused to patients...
Due to the many advances in the areas of biotechnology and medicine, the need for long-
term storage and stabilization of biological materials is rapidly increasing. The field of
biopreservation is attempting to address these issues by finding ways to maintain the integrity and
functionality of proteins, cells, and organs...
Gram positive cocci isolated from Oregon wines
were characterized and their relative malate fermentation
abilities compared. They were heterofermentative,
catalase negative, and facultatively anaerobic. Glucose,
fructose, cellobiose, maltose, ribose, trehalose, salicin
and esculin were fermented by all strains. Arabinose was
fermented weakly but lactose, raffinose, sucrose, xylose,
rhamnose, mannose and...
Spray drying has been widely used in food science for long-term storage of products such as powdered milk, and it has recently been used for preservation of more temperature sensitive products such as human blood plasma and single-cell organisms. However, there are no previous reports of successful spray drying of...
Cryopreservation of human red blood cells (RBCs) in the presence of 40% glycerol
allows a shelf-life of 10 years, as opposed to only 6 weeks for refrigerated
RBCs. Nonetheless, cryopreserved blood is rarely used in clinical therapy, in part
because of the requirement for a time-consuming (~1 h) post-thaw wash...
The leaching of water soluble phenolic compounds from
cold stressed Rhododendron leaves was found to be a reliable
indicator of tissue damage. In sublethally stressed leaf
tissues, more and novel phenolic compounds appeared and were
hypothesized to be precursors of cell wall components such
as lignin and suberin. Subsequent radiolabeling...
The major portion of Pacific whiting (PW) is commercialized in the form of frozen surimi. Alternative products for PW were investigated focusing on fresh surimi and texturized meat from PW mince. Fresh surimi is made without additives and kept refrigerated instead of frozen. Texturized meat is a meat-like product made...
The heart rate, oxygen consumption, survivorship, and 1-lactate
accumulation were determined in the intertidal limpets Collisella pelta
and Collisella digitalis in relation to laboratory desiccation at 15°C
or 30°C. Water loss was computed as a percentage of total initial body
water (wet weight - dry weight). Although water was lost...
Phage-insensitive Streptococcus cremoris starter strains were
selected by assaying cheese whey against potential starter
strains. Six strains were selected and characterized for continual
use in cheesemaking. Upon phage-infection, strains were removed
from the blend. Cheesemaking continued with remaining strains. A
phage-insensitive, fast-acid-producing mutant of the infected
strain was isolated and...
Zebrafish (Danio rerio) are one of the most commonly used animal models in biomedical research. Zebrafish resource facilities, like the Zebrafish International Resource Center (ZIRC) in Eugene, Oregon, are the main providers and keepers of numerous zebrafish wild-type, mutant, and transgenic lines. Although ZIRC maintains live zebrafish at various life...
Commercial vats of Chardonnay and Pinot Noir were
inoculated during yeast fermentation with starter cultures
containing 10⁷ - 10⁹ cfu/ml of the malolactic bacteria
Leuconostoc oenos Erla and Ey2d in lots ranging from 55 to
4,000 gallons. Chardonnay inoculated with Ey2d completed
MLF in an average of 56 days at...
This research investigated the development of a novel canine model to study preeclampsia. Normal canine placental development has morphologic and histologic similarities to the shallow trophoblast invasion occurring with preeclampsia in humans, which makes the dog a particularly good choice for modeling this disease and will be an improvement on...
Experimental evolution is a tool that allows us to measure changes in populations over time in controlled, novel environments. Microbial evolution experiments use cryopreservation – storage at -80°C in glycerol media – to archive experimental populations. Research with Escherichia coli suggests that cryopreservation conditions can affect cell viability and that...
Two multiple strain lactic streptococcal starter concentrates
were examined to determine whether or not elimination of one of the
three component strains, by addition of its specific phage, resulted
in disproportionate inhibition of acid production (strain dominance).
Both starters showed dominance when tested three to four weeks after
freezing and...
Spring frost damage to pears in the Pacific Northwest frequently
results in substantial crop losses. This research was undertaken to
investigate the effects of frost on pear buds, flowers, and fruit
through controlled freezing tests and field studies in order to better
understand the frost phenomenon and refine frost protection...
Cryoprotection in stabilized mince from Pacific whiting (Merluccius productus) was investigated by monitoring changes in physical and chemical properties during 32 weeks of frozen storage. The effects of 4 different cryoprotectants were evaluated by torsion test, color analysis, extractability of salt soluble proteins, and formation of dimethylamine (DMA) and 2-thiobarbituric...
Back pain is the leading cause of disability worldwide, entailing a significant socioeconomic impact. A primary source of back pain can be attributed to intervertebral disc (IVD) degeneration allowing nerve ingrowth, facet joint arthritis, disc bulging, and osteophyte formations that press on nearby nerve roots or the spinal cord. While...
Background: Little is known about the potential of Brachypodium distachyon as a model for low temperature stress
responses in Pooideae. The ice recrystallization inhibition protein (IRIP) genes, fructosyltransferase (FST) genes, and
many C-repeat binding factor (CBF) genes are Pooideae specific and important in low temperature responses. Here
we used comparative...
A rapid and sensitive HPLC method for quantifying both the
reduced and oxidized forms of glutathione simultaneously in woody
plant tissues was developed. This method was applicable to a variety
of woody plant species and different tissues. Total glutathione
content in the bark tissue of seven of the woody plant...
Hydrogen cyanamide (CY) has been used to break rest
in temperate crops, but levels used to overcome rest are
sometimes toxic to plants. The rest breaking effect of CY
is thought to involve peroxide metabolism. Inhibition of
catalase by CY has been proposed as a mechanism for
overcoming dormancy in...
Cryoprotectants (CPAs) such as glycerol and dimethyl sulfoxide (DMSO) are commonly used during cryopreservation of cell based therapeutics. Although these additives are beneficial during freezing, it is often desirable to remove them before infusion into a patient. Currently, the most common method for CPA removal is by centrifugation. This method...