Levels of activity of glucose-6-phosphate- , 6-phosphogluconate- , and isocitric- dehydrogenase were measured in the
soluble and particulate fractions of heart, brain and liver at
several ages throughout the development of the chick embryo.
Compared to isocitric dehydrogenase, levels of glucosesphosphate-
, and 6-phosphogluconate- dehydrogenase are generally
low. This suggests...
Four-fold partially purified glucose-6-phosphate dehydrogenase
(G-6-PDH) from the obligately psychrophilic marine bacterium
Vibrio marinus was effected by thermally induced leakage of the
enzyme, ammonium sulfate fractionation and column chromatography
on Sephadex G-200 gel.
The effects of temperature, pressure, salinity, and pH were
determined for the various degrees of enzyme purity....
The cytoplasmic isozyme of malate dehydrogenase (s-MDH) has been isolated from brine shrimp nauplii to a state of high purity with a protocol involving ammonium sulfate fractionation and chromatography on DEAE-cellulose, followed by sequential passes over cellulose phosphate columns at pH 5.8 then 5.0. The purified enzyme is free of...
The purposes of this investigation were to measure the relative
levels of enzymatic and respiratory metabolism of some vertically
migrating mesopelagic fishes collected from the ocean waters off
Oregon and to modify and adapt an enzyme method for use as a measure
of metabolic activity at sea with a minimum...
Butane-grown Pseudomonas butanovora oxidized butane by a soluble butane monooxygenase through the terminal pathway yielding 1 -butanol as the predominant product. Alcohol dehydrogenases (ADHs) involved in butane oxidation in P. butanovora were purified and characterized at the biochemical, genetic and physiological levels. Butane-grown P. butanovora expressed a type I soluble...
This work sought to characterize a link between Vitamin C and nitroglycerin at the level of enzymatic bioactivation. Before we could proceed, we needed to develop a method that sensitively and selectively identified the product of bioactivation, nitrite. We successfully adapted traditional diaminonaphthalene derivatization with the use of stable isotope...
A method of measuring the dehydrogenase activity of sediments at lOC was
calibrated by direct microcalorimetry at the same temperature. Field
measurements of dehydrogenase activity of sediments in Lake Washington
were converted to rates of metabolic heat release by means of the significant
regression of dehydrogenase activity on metabolic heat...
