Various flavonoid-rich plant extracts have shown efficacy in the treatment of diabetes. Although it is often assumed that dietary flavonoids exert biological antioxidant effects, it is possible that the observed effects work in carbohydrate metabolism. In this study we evaluated the effect of catechins and catechin containing extracts on α-amylase...
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BalzFrei
Various flavonoid-rich plant extracts have shown efficacy in the treatment of
Various flavonoid-rich plant extracts have shown efficacy in the treatment of diabetes. Although it is often assumed that dietary flavonoids exert biological antioxidant effects, it is possible that the observed effects work in carbohydrate metabolism. In this study we evaluated the effect of catechins and catechin containing extracts on α-amylase...
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_______________________________________________________________________________________________
BalzFrei
Various flavonoid-rich plant extracts have shown efficacy in the treatment of
Endothelial activation with increased expression of cellular adhesion molecules, chemokines and pro-inflammatory cytokines critically contributes to vascular inflammation and the initiation and progression of atherosclerosis. By affecting redox-sensitive cell signaling pathways and transcription factors, redox-active transition metal ions, such as copper and iron, may play an important role in these...
Atherosclerosis is the underlying cause of ischemic heart disease and stroke and is the leading cause of death worldwide, especially in developed countries. As an inflammatory disease of arteries in a hyperlipidemic milieu, expression of adhesion molecules, such as endothelial-leukocyte adhesion molecule-1 (E-selectin) and intercellular adhesion molecule-1 (ICAM-1), on endothelial...
On behalf of the Linus Pauling Institute, I am pleased to welcome you to this conference and, in particular, to the sessions on the Health Benefits of Seafood Consumption. It is our pleasure to be a co-sponsor of these sessions and I look forward to the presentations by the distinguished...
Oxidative stress is the major driving force behind the aging process and many age-related diseases. However, direct experimental evidence of whether antioxidants, such as ascorbate (AA) and lipoic acid (LA) can slow the progression of aging process and/or reduce risks of developing degenerative disease is largely absent. This suggests a...
In this study we investigated the role of astragaloside IV (AS-IV), one of the major active constituents purified from the Chinese
medicinal herb Astragalus membranaceus, in LPS-induced acute inflammatory responses in mice in vivo and examined possible
underlying mechanisms. Mice were assigned to four groups: vehicle-treated control animals; AS-IV-treated animals...
Research progress to understand the role of vitamin C (ascorbic acid) in human health has been slow in coming. This is predominantly the result of several flawed approaches to study design, often lacking a full appreciation of the redox chemistry and biology of ascorbic acid. In this review, we summarize...
The recommended dietary allowance (RDA) of vitamin C has traditionally been based on the prevention of the vitamin C deficiency disease, scurvy. While higher intakes of vitamin C may exert additional health benefits, the limited Phase III randomized placebo-controlled trials (RCTs) of vitamin C supplementation have not found consistent benefit...
We reviewed recent scientific evidence regarding the effects of MVM [multivitamin/mineral] supplements on risk of chronic diseases, including cancer, cardiovascular disease, and age-related eye diseases. Data from both randomized controlled trials (RCTs) and observational, prospective cohort studies were examined. The majority of scientific studies investigating the use of MVM supplements...
Inhibiting pancreatic lipase activity is an effective means of reducing fat absorption, which may have a role in combating obesity by limiting energy intake. The use of pharmaceutical lipase inhibitors has been limited due to the prevalence of undesirable side effects. Polyphenols represent an alternative strategy to inhibit digestive enzymes...
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Michelle M. Hess, Alexander J. Michels, and BalzFrei
Linus Pauling Institute, Oregon State University
Inhibiting pancreatic lipase activity is an effective means of reducing fat absorption, which may have a role in combating obesity by limiting energy intake. The use of pharmaceutical lipase inhibitors has been limited due to the prevalence of undesirable side effects. Polyphenols represent an alternative strategy to inhibit digestive enzymes...
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and Tea Extracts and Their Primary Catechins in vitro
Michelle M. Hess, Alexander J. Michels, and Balz
Inhibiting pancreatic lipase activity is an effective means of reducing fat absorption, which may have a role in combating obesity by limiting energy intake. The use of pharmaceutical lipase inhibitors has been limited due to the prevalence of undesirable side effects. Polyphenols represent an alternative strategy to inhibit digestive enzymes...
The vascular endothelium is a single cell layer that lines the lumen of the entire vasculature. It is the site of synthesis of nitric oxide (NO), a vasodilatory compound synthesized by endothelial nitric oxide synthase (eNOS). NO causes intracellular calcium sequestration of the vascular smooth muscle cells, relaxing and dilating...
Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
Ceramides are a group of lipids in the sphingolipid family that are potent cell signaling molecules. Elevations in ceramide levels above the norm generally lead to apoptosis. Evidence from in vitro studies suggests that accumulation of ceramides within mitochondria leads to dysfunction of the mitochondria. This includes inhibition of the...
Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2 or Nrf2) is the primary transcription factor in cellular defense against oxidative and xenobiotic stresses in higher eukaryotes. This basic leucine zipper transcription factor regulates over 200 antioxidant, detoxification, and lipid metabolizing genes by binding to the Antioxidant Response Element (ARE; a...
Indole-3-carbinol (I3C) is a naturally occurring plant alkaloid, found in significant concentrations in cruciferous vegetables such as broccoli and Brussels sprouts. I3C is an unstable compound that undergoes rapid oligomerization in an acidic environment to form higher order condensation products (I3C-ACPs), such as 3-3'-diindolylmethane (DIM). Both I3C and DIM are...
Glutathione (GSH) is the predominant low molecular weight thiol antioxidant
in liver tissue. GSH plays an important role in maintaining the intracellular thiol redox
ratio as well as detoxification of electrophiles and xenobiotics. Aging leads to a significant decline (35%; P ≤ 0.05) in hepatocellular GSH levels. Using young (2-4...
The purposes of this study were to: 1) determine in what ways incorporating active engagement into a traditional lecture chemistry course contributed to students' science identity and their motivation to learn the science, and 2) determine how the development of a science identity could be measured through the use of...
Protein disulfide isomerase (PDI), a member of the thioredoxin superfamily, contains two domains with significant sequence homology to the active sites in thioredoxin. PDI facilitates the folding of nascent proteins in the endoplasmic reticulum (ER), binds hormones and Ca²⁺, catalyzes the glutathione dependent reduction of dehydroascorbate, serves as a major...
Hallmarks of aging include the accumulation of aberrant proteins and a lower resistance to stresses. Because the endoplasmic reticulum (ER) functions to fold proteins and possesses unique stress sensing pathways, the central hypothesis of this dissertation is that the ER is a target of cellular aging and significantly underlies these...
The effect of three different weed management strategies, non-weeding, hand weeding, and weed mat, were examined on physicochemical, sugar profile, and antioxidant properties of two cultivars of blackberry (Rubus spp), ’Marion’ and ’Black Diamond’ harvested at three time intervals during the 2012 season. Sensory analysis on flavor intensity of six...
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
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, Praveen Rajendran2, Mohaiza W Dashwood2, Mark A Levine4, Alexander Michels2,
Balz Frei2 and Roderick H
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
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, p53, tubulin)
Dr. Rod Dashwood and lab (IBT)
Dr. BalzFrei, Dr. Alex Michels (LPI) Dr.
Mark
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
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)
Dr. BalzFrei, Dr. Alex Michels (LPI) Dr. Mark Levine (NIH)
Helen P. Rumbel Fellowship
OSU Honors
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
Aim: There is growing interest in pharmacologic ascorbate (Asc) and its therapeutic properties (Levine et al. Adv Nutr 2011;2:78). W e examined cell viability, histone deacetylase (HDAC) expression, and related protein modifications in cancer versus non- cancer colon epithelial cells following exposure to Asc.
Methods: MTT assays were conducted in...
Decline in cardiac pump function is a hallmark of aging where mitochondrial decay is an important underlying cause. Although certainly multifactorial in nature, both dysfunction of the machinery involved in the chemiosmotic process of energy transduction and lower capacity to maintain fatty acid-driven respiration are identified as intrinsic factors of...
Aerobic organisms have evolved many sensory mechanisms that allow
response to oxidants in the environment. One area of interest is the relationship
between the activity of the tumor suppressor protein p53 and the redox state of
thioredoxin. Human p53 activity is severely compromised in budding yeast lacking
thioredoxin reductase. Evidence...
Mutations to superoxide dismutase were the first proven cause of Lou
Gehrig’s disease (amyotrophic lateral sclerosis; ALS) implicating superoxide in
the selective death of motor neurons that characterizes ALS. Nitric oxide
competes effectively with superoxide dismutase for superoxide to form the
powerful oxidant peroxynitrite. Endogenous formation of peroxynitrite can kill...
This talk provides an overview of Linus Pauling's life and work, discusses the background and composition of the book "Visions of Linus Pauling," and touches on some themes in the history of the Linus Pauling Institute of Science and Medicine.
Seasonality is defined as a change in mood and behavior with the seasons. Research shows there is a possible connection between vitamin D levels and mood (Murphy & Wagner, 2008; Lansdowne & Provost, 1998). Given that vitamin D is produced when skin is exposed to sunlight and varies with sun...
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Performance, Good Samaritan Hospital Foundation for funding; and Adrian Gombart and BalzFrei of the Linus
Seasonality is defined as a change in mood and behavior with the seasons. Research shows there is a possible connection between vitamin D levels and mood (Murphy & Wagner, 2008; Lansdowne & Provost, 1998). Given that vitamin D is produced when skin is exposed to sunlight and varies with sun...
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Adrian Gombart and BalzFrei of the Linus Pauling Institute, and David Zava of ZRT Laboratory for their
To determine whether extreme endurance exercise induces lipid peroxidation, we studied 14 athletes (5 females: 9 males) during a 50 km ultramarathon (trial 1) and during a sedentary protocol (trial 2) one month later. At dinner the evening before the race (or sedentary trial), subjects consumed vitamin E labeled with...
The Conference Program for IIFET, held July 10-14 in Corvallis, Oregon. Program lists the sessions by date and time and includes the session titles, speakers and paper titles.
Blackberry (Rubus sp.) fruit are a good source of phenolic compounds and anthocyanins, and are consumed in fresh and processed forms. Though organic products become more popular, limited information is available about how different organic production methods affect the post-harvest quality of blackberry fruit. The objective of this project was...
To determine if 6 weeks prior supplementation with vitamins E and C
could alleviate exercise-induced DNA damage, prevent lipid peroxidation and
inflammation, slow the rate of vitamin E utilization and/or attenuate muscle
damage, 22 subjects (11 females: 11 males) were studied during a 50 km (32
mile) ultramarathon. Subjects were...
It has been suggested that cellular damage from oxygen radicals is one of the processes leading to cardio-vascular disease and cancer. Natural antioxidants prevent uncontrolled oxidative reactions by decreasing molecular oxygen levels, scavenging chain-initiating and chain-propagating free radicals, chelating metals, or decomposing peroxides. Beer is rich in antioxidants, derived both...
The objective of this project was to investigate the effects of refrigeration storage and processing technologies on the quality and nutraceutical benefit of blackberries. 'Marion' and 'Evergreen', the two major blackberry varieties in Oregon, were evaluated in this study. For refrigeration storage, fresh fruit were packed in clam-shell containers right...
Purple tomatoes (Lycopersicon esculemtum) were bred by incorporating
genes that result in anthocyanin production in the skin of fruits. These genes
originally came from related wild species as no naturally occurring genes encoding
fruit anthocyanin expression are known within the cultivated species. By
combining Aft or Abg with atv or...
The objective of this work is to add to the body of translational data between high dose animal model research and the environmentally relevant human metabolism of the persistent pollutant dibenzo[def,p]chrysene (DBC). We furthered the knowledge of gene/exposure interactions by determining the carcinogenesis risk based on Cyp1b1 genotype following in...
Targeting the epigenome, including the use of histone deacetylase (HDAC) inhibitors, is a novel strategy for cancer chemoprevention. Sulforaphane (SFN), a compound found at high levels in broccoli and broccoli sprouts, is a potent inducer of Phase 2 detoxification enzymes and inhibits tumorigenesis in animal models. SFN also has a...