Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We employed activation tagging to isolate a dominant mutation affecting release from dormancy, and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and...
Full Text:
dormancy in poplar
trees
YordanS. Yordanov
1
, Cathleen Ma
2
, Steven H. Strauss
2
and Victor B
Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We employed activation tagging to isolate a dominant mutation affecting release from dormancy, and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and...
Trees from temperate latitudes transition between growth and dormancy to survive dehydration and freezing stress during winter months. We employed activation tagging to isolate a dominant mutation affecting release from dormancy, and identified the corresponding gene EARLY BUD-BREAK 1 (EBB1). We demonstrate through positioning of the tag, expression analysis, and...
Eukaryotes contain a diversified set of small RNA-guided pathways that control genes, repeated sequences, and viruses at the transcriptional and posttranscriptional levels. Genome-wide profiles and analyses of small RNAs, particularly the large class of 24-nucleotide (nt) short interfering RNAs (siRNAs), were done for wild-type Arabidopsis thaliana and silencing pathway mutants...
ABSTRACT
Eukaryotes produce functionally diverse classes of
small RNAs (20–25 nt). These include microRNAs
(miRNAs), which act as regulatory factors during
growth and development, and short-interfering
RNAs (siRNAs), which function in several epigenetic
and post-transcriptional silencing systems. The
Arabidopsis Small RNA Project (ASRP) seeks to characterize
and functionally analyze the...
Budburst is a key adaptive trait that can help us understand how plants respond to a changing climate from the molecular to landscape scale. Despite this, acquisition of budburst data is constrained by a lack of information at the plant scale on the environmental stimuli associated with the release of...
Bud break is a key adaptive trait that can help us understand how plants respond to a changing climate from the molecular to landscape scale. Despite this, acquisition of bud break data is currently constrained by cost, scale, and a lack of information at the plant scale on the environmental...
The effect of high pressure processing (HPP) pre-treatments at three pressure levels (150, 300 and 450 MPa) and holding times (0, 2.5 and 5 min) on the activity of key enzymes in Atlantic mackerel (Scomber scombrus) muscle was studied after HPP treatments and during frozen storage. Acid phosphatase, cathepsins (B...
Multicellular eukaryotes produce small RNA molecules (approximately 21–24 nucleotides) of two general types, microRNA (miRNA) and short interfering RNA (siRNA). They collectively function as sequence-specific guides to silence or regulate genes, transposons, and viruses and to modify chromatin and genome structure. Formation or activity of small RNAs requires factors belonging...
Bloomy rind cheeses, including Camembert and Brie type cheeses, are highly susceptible to contamination by environmental pathogens during their manufacture and ripening. These cheeses undergo many physiochemical changes during ripening that provides these pathogens with a suitable environment to grow. One example of this change is an increase of pH...
Recalcitrance to transformation makes genetic engineering of many valuable plants infeasible for practical use. Transitory host modification during transformation using transgenes offers a possible means of overcoming this obstacle. In prior work in our laboratory, the genes GA20ox7 and EBB1 were found to increase regeneration in vitro. In this project,...
Full Text:
transformation of plants.
Annu Rev Microbiol 42:575-606
Brencic, A., Eberhard, A., & Winans, S. (2004
The assessment of enzymatic activity on Atlantic horse mackerel (Trachurus trachurus) during frozen storage was carried out in samples pre-treated by high pressure processing (HPP) combinations of 150, 300 and 450 MPa with 0, 2.5 and 5 min holding time (untreated samples were used as controls). The activities of four...
To survive winter, many perennial plants become endodormant, a state of suspended growth maintained even in favorable growing environments. To understand vegetative bud endodormancy, we collected paradormant, endodormant, and ecodormant axillary buds from Populus trees growing under natural conditions. Of 44,441 Populus gene models analyzed using NimbleGen microarrays, we found...
Full Text:
., Dharmawardhana, P., Priest, H. D., Mockler, T. C., &
Strauss, S. H. (2015). Extensive transcriptome changes
The fate of antimicrobials entering the aquatic environment is an increasing concern for researchers and regulators, and recent research has focused on antimicrobial contamination from point sources, such as wastewater treatment facility outfalls. The terraccumulation of antimicrobials and mobility in diffuse pollution pathways should not be overlooked as a contributor...
Epigenetic mechanisms are important for control of plant development, and may play a particularly important role in trees given their long life cycles and distinctive and stable tissue types. To help understand the role of epigenetics in tree development, we produced transgenic poplars with reduced activity of the DDM1 genes,...
Humans are remarkably efficient in learning by interacting with other people and observing their behavior. Children learn by watching their parents’ actions and mimic their behavior. When they are not sure about their parents demonstration, they communicate with them, ask questions, and learn from their feedback. On the other hand,...
Wildtype sunflower typically produces 12-24% oleic acid (18:1) and 70-82%
linoleic acid (18:2). High-oleic sunflower, by contrast, produces up to 80-94% oleic
acid. The monounsaturated oleic acid has a greater oxidative stability than the
polyunsaturated linoleic acid, predominant in wildtype sunflower, and has greater
nutritional benefits than polyunsaturated and saturated...
RNA silencing pathways are required for a wide variety of processes in most
eucaryotes. In plants, small-interfering RNA (siRNA) arising from transposons and other
repetitive sequences is associated with heterochromatin formation and maintenance.
MicroRNAs and trans-acting siRNAs encoded at discrete loci function as negative regulators of
gene expression by triggering...
Transition metal oxides are an important class of materials for the wide variety optical, electrical, dielectric, magnetic, and thermal properties observed. Their unique structure-property relationships allow for property tuning and often provide insight into the fundamentals of chemistry. The work in this thesis was focused on the design, synthesis and...
It is uncertain how predicted changes in climate will impact vegetation responses and plant species’ distributions because the physiological mechanisms underlying thresholds for damage are not well understood, and responses to stress vary by functional type and developmental stage. Thus, it is crucial to investigate physiological responses to heat and...
The potential for nanomedicine to reduce toxicity and improve therapeutic response to radiation is vast. Clinically, this potential has thus far only begun to be realized with the recent success of the radioenhancing nanoparticle, NBTXR3 by Nanobiotix. Radioenhancing nanoparticles, however, represent only a small class of nanomaterials that may be...
European hazelnut (Corylus avellana L.) is an important crop Oregon's Willamette Valley, producing 99% of the hazelnuts grown in North America and brings over US $60 million dollars to the region annually. Hazelnuts are rich in fiber and vitamins, as well in demand by consumers due to their popularity as...
The fusion reaction ²⁰⁸Pb (⁵⁰Ti, xn) ²⁵⁸⁻x Rf (x = 1-3) was studied to determine PCN (probability that the mononucleus system evolves to form a compound nucleus inside the fission saddle point) and to establish the value of Wsur (survival probability) for the given reaction experimentally. The fission excitation function...