Replication of plasmid molecules containing a single
adenovirus terminal sequence and specially arranged
inverted repeat sequences were studied. When the plasmids
were linearized to expose the adenovirus terminal sequence
and transfected into 293 cells with helper adenovirus DNA,
a larger molecule was detected. The new molecule
contained adenovirus terminal sequences...
Replication of plasmid molecules containing either a left-end or a
right-end adenovirus origin was investigated. When the plasmids were
linearized to expose their respective adenovirus termini, mixed, and
transfected with helper adenovirus DNA into the human 293 cell line, a
new molecule was detected. The new molecule was the size...
Fifteen adenoviruses from cattle, sheep, horses, and llamas were studied by virus neutralization to determine their degree of antigenic similarity. Prototype viruses included bovine adenoviruses species 1-8, ovine adenoviruses species 5 and 6, and equine adenovirus species 1. Unclassified viruses that were compared to the prototype viruses were isolated from...
Four bovine adenovirus type 3 isolates, recovered from different areas in Oregon and Washington, were compared antigenically with the original prototype strain which was isolated in England. The fluorescent foci assay method was used to quantitatively establish viral titers. A serum-viral neutralization test was performed with a standard dose of...
Six 3 to 4 weeks old, cesarian-derived lambs were inoculated with ovine an adenovirus isolate 475N. Inoculated lambs showed moderate clinical signs of respiratory distress, conjunctivitis, and loose feces during the 10-day observation period. Virus was detected from nasal and conjunctival swabs starting on postinoculation day (PID) 2.
Virus was...
An enzyme-linked immunosorbent assay was developed to
measure specific antibody response in bovine sera to bovine
adenovirus type 3 (BA3), an etiologic agent of respiratory
disease causing economic losses annualy to the cattle
industry. Observed endpoint titers were determined using
the intersection point from optical density values of
serially diluted...
Adenovirus initiates strand-displacement DNA replication from
origins located in identical inverted terminal repetitions (ITRs).
Panhandle structures, formed by base-pairing between ITRs on the
displaced strands, have been proposed as replication intermediates for
complementary strand synthesis. Plasmid molecules containing a single
adenovirus terminal sequence and specially arranged inverted repeat
sequences were...
The structure of the nucleoprotein core of adenovirus has been
investigated. Using a combination of biophysical techniques and
nuclease digestion I have determined that the adenovirus core, as
isolated with pyridine, shares several physical properties with cellular
chromatin. The most notable difference is the lack of a nucleosome
repeat in...
The work in this thesis has provided conclusive genetic evidence
that "panhandle" intermediates form during adenovirus replication.
Adenovirus chromosomes lacking 51 by from their left -hand termini
are infectious and capable of regenerating the missing origin
sequence. Yet if an entire inverted terminal repeat is removed, the
adenovirus chromosome is...