Monoclonal antibodies (Mabs) were produced against the
myxosporean parasite, Ceratomyxa shasta, and were characterized
by indirect immunofluorescent techniques, western blot analysis,
and immunoelectron microscopy. The resulting Mabs reacted
specifically with the trophozoite and sporoblast stages of the
parasite, but not with the mature spore, indicating that certain
antigens are stage-specific....
Erythrocytic inclusion body syndrome (EIBS), a viral disease
of salmon, was investigated. Reliable artificial infections were
established in coho salmon (Oncorhynchus kisutch) via
intraperitoneal injection of homogenized kidney, spleen, and blood
from naturally infected animals. By examining the exposed fish
periodically for viral inclusion bodies and hematocrits, the disease
progression...
The virulence of selected isolants of infectious hematopoietic
necrosis virus (IHNV) were differentiated and correlated with
electrophoretic mobilities of virion structural proteins by sodium
dodecylsulfate polyacrylamide gel electrophoresis. A type 1
electropherotype of IHNV was more virulent for kokanee salmon
(Oncorhynchus nerka) and a type 2 strain more virulent for...
Selected secreted and cellular virulence factors of Aeromonas
salmonicida were examined. A protocol was developed for the
separation of two secreted proteases (P1 and P2 protease), and
a trout erythrocyte specific hemolysin (T-lysin) from
supernatants of cultures of the bacterium. Distinctions between
the proteases were demonstrated using molecular weight
determinations,...
Erythrocytic inclusion body syndrome (EIBS) was artificially
established in selected stocks of juvenile fall and spring chinook
salmon (Oncorhynchus tshawytscha), chum salmon (0. keta), coho
salmon (0. kisutch), Atlantic salmon (Salmo salar), and rainbow
trout (0. mykiss). Adult spring chinook salmon were also
artificially infected with the EIBS virus. Adult...
Three closely related isolates belonging to the A₁ serotype of infectious pancreatic
necrosis virus (IPNV) were selected for comparison, to provide insight into the nature of
variation in the virulence of IPN viruses. Brook trout fry (Salvelinus fontinalis) were
experimentally infected with the three isolates by immersion. Cumulative mortalities over...