Cryopreservation of pollen offers a simple and effective method of
long-term pollen storage. Pollen may be stored in liquid nitrogen (LN)
for many years without loss of its essential capabilities to pollinate,
fertilize, and set normal fruit and seed when used in plant breeding,
for controlled pollinations, or for the...
Effective methods for long-term preservation of cord red blood cells (RBCs) are needed to ensure a readily available supply of RBCs to treat fetal and neonatal anemia. Cryopreservation is a potential long-term storage strategy for maintaining the quality of cord RBCs for the use in intrauterine and neonatal transfusion. However,...
Cryopreservation nearly universally depends on the equilibration of cells and tissues with high concentrations of permeating chemicals known as cryoprotective agents, or CPAs. Despite their protective properties, CPAs can cause damage as a result of osmotically-driven cell volume changes, as well as chemical toxicity. In this study, we have used...
Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s ability to form a stable...
Improved recovery of cryopreserved meristems and calli from in
vitro currant (Ribes aureum Pursh and R. ciliatum Humb. & Bonpl.) plants
was obtained by two-hour pretreatment with sucrose, proline, abscisic
acid-responsive protein (RABP), and bovine serum albumin (BSA)
solutions. Meristems and calli cold-acclimatized for one week were
immersed in pretreatment...
Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used...
Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to...
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...