The purpose of this study was to evaluate the impact sediment has on the survival of
bacteria in a typical rangeland stream. This information is an important component in
determining a time integrated prediction of bacterial numbers in the stream sediment and
overlying water. Bacterial survival in stream is a...
The effect of canopy orientation on fruiting performance of 'Beurre
d'Anjou' pears (Pyrus communis L.) and postharvest urea spray on ovule
longevity and fruit set of 'Doyenne du Comice' pears were studied.
Hedgerows of 'Beurre d'Anjou' pear trees planted in 1969 in two
orientations north-south (N-S) and east-west (E-W) were...
The WWC family consists of three proteins‐ KIBRA/WWC1, WWC2, and WWC3. The three proteins share a similar primary structure and interact with a common set of proteins. Each paralog contains two WW domains which recognize proline‐rich sequences of the type PPxY (P is proline, Y is tyrosine, x is any...
Ultrafast spectroscopy has recently gained momentum as a powerful, noninvasive characterization toolset capable of studying a diverse array of samples with applications in chemistry, physics, biology, engineering, and more. Two popular ultrafast spectroscopic techniques include femtosecond transient absorption (fs-TA) and femtosecond stimulated Raman spectroscopy (FSRS). The latter of these techniques...
In the past decades, femtosecond stimulated Raman spectroscopy (FSRS) has been gaining tremendous popularity in fundamental sciences stemming from chemistry to biology. It is capable of capturing both equilibrium and non-equilibrium structural information across a broad range of timescales with simultaneously high temporal and spectral resolutions. Femtosecond transient absorption spectroscopy...
Ultrafast spectroscopy can exploit the structure-function relationship of chemical- and biological-based systems. More specifically, femtosecond stimulated Raman spectroscopy (FSRS) provides ground- and excited-state vibrational information with simultaneously high spectral (spatial) and temporal resolutions. The electronic counterpart, femtosecond transient absorption (fs-TA), complements FSRS data analysis as part of a comprehensive approach...
Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Soils and other resource programs in both public land management agencies and private industry are continually being adapted to the challenges of evolving knowledge and experience in the field of forestry. This dissertation explores new ways of thinking about and using soils information in forest planning and management, with a...
Photoactivated biomedical tools like fluorescent biosensors and optogenetic proteins have increased in popularity due to the precision targeting and activation used for in vivo applications. In nature, the initially discovered parent proteins exhibit properties such as fluorescence quantum yield (FQY), fluorescence color, and photoswitching dynamics that are unfavorable in mammalian...
Femtosecond stimulated Raman spectroscopy (FSRS) is a powerful ultrafast technique which can track photoinduced excited state structural events on femtosecond (fs) to picosecond (ps) timescales. In addition to high temporal and spectral resolutions, FSRS provides a broad spectral window from ca. 100—2000 cm-1 for detection, enabling the direct mapping of...