The effect of canopy orientation on fruiting performance of 'Beurre
d'Anjou' pears (Pyrus communis L.) and postharvest urea spray on ovule
longevity and fruit set of 'Doyenne du Comice' pears were studied.
Hedgerows of 'Beurre d'Anjou' pear trees planted in 1969 in two
orientations north-south (N-S) and east-west (E-W) were...
In this dissertation, my research work on excited state dynamics of small organic molecules in solutions is presented. Using femtosecond stimulated Raman spectroscopy (FSRS) as the main experimental tool, the excited state hydrogen-bonding dynamics (ESHBD) of Coumarin 102, a common laser dye molecule, in ethanol solution is examined with <150...
This biological assessment (BA) documents the potential effects of existing Forest Service (FS) Land and Resource Management Plans (LRMPs), or 'Forest Plans' and Bureau of Land Management (BLM) Land Use Plans (LUPs) on the Canada Lynx (Lynx canadensis), hereafter referred to as the lynx. LRMPs and LUPs are collectively referred...
In the past decades, femtosecond stimulated Raman spectroscopy (FSRS) has been gaining tremendous popularity in fundamental sciences stemming from chemistry to biology. It is capable of capturing both equilibrium and non-equilibrium structural information across a broad range of timescales with simultaneously high temporal and spectral resolutions. Femtosecond transient absorption spectroscopy...
Ultrafast spectroscopy can exploit the structure-function relationship of chemical- and biological-based systems. More specifically, femtosecond stimulated Raman spectroscopy (FSRS) provides ground- and excited-state vibrational information with simultaneously high spectral (spatial) and temporal resolutions. The electronic counterpart, femtosecond transient absorption (fs-TA), complements FSRS data analysis as part of a comprehensive approach...
Fluorescent proteins (FPs) are luminescent biomolecules that emit characteristic hues upon irradiation. A group of calmodulin (CaM)-green FP (GFP) chimeras have been previously engineered to enable the optical detection of calcium ions (Ca²⁺). We investigate one of these genetically encoded Ca²⁺ biosensors for optical imaging (GECOs), GEM-GECO1, which fluoresces green...
Photoactivated biomedical tools like fluorescent biosensors and optogenetic proteins have increased in popularity due to the precision targeting and activation used for in vivo applications. In nature, the initially discovered parent proteins exhibit properties such as fluorescence quantum yield (FQY), fluorescence color, and photoswitching dynamics that are unfavorable in mammalian...
The goal of this study was to develop an understanding of the nature of surge wave loading on
wood framed residential structures for a variety of building configurations and test conditions.
The objectives of the study were: (1) to measure forces on a 1/6th scale wood framed
residential structure, (2)...
Femtosecond stimulated Raman spectroscopy (FSRS) is a powerful ultrafast technique which can track photoinduced excited state structural events on femtosecond (fs) to picosecond (ps) timescales. In addition to high temporal and spectral resolutions, FSRS provides a broad spectral window from ca. 100—2000 cm-1 for detection, enabling the direct mapping of...
In this dissertation, excited state proton transfer (ESPT) and its inhibition in solution and protein environments are revealed using both femtosecond transient absorption (fs-TA) spectroscopy and femtosecond stimulated Raman spectroscopy (FSRS). Using a tunable Raman pump to enhance transient vibrational features of the photoacidic chromophore HPTS in methanol and methanol...