Plasmid transformation of chlamydiae has created new opportunities to investigate host–microbe interactions during chlamydial infections; however, there are still limitations. Plasmid transformation requires a replicon derived from the native Chlamydia plasmid, and these transformations are species-specific. We explored the utility of a broad host-range plasmid, pBBR1MCS-4, to transform chlamydiae, with...
Managers of forest and plantation ecosystems are encountering growing problems involving plant pathogens and the expanding geographic ranges of these pathogens. Historically unexposed stands are exceptionally vulnerable when a non-native pathogen is introduced because these pathogens can cause devastating disease in a host population that lacks co-evolved resistance mechanisms. One...
The purpose of this study is to develop
a workable plant layout
evaluation technique incorporating adequate
definitive measures of
intangible plant layout considerations.
A summary and critique is
given of existing evaluation methods. A
quantitative evaluation index
(QEI) is proposed to remedy the described
deficiencies.
The proposed index can be...