High-throughput culturing (HTC) consisting of extinction culturing in autoclaved seawater has led to the isolation and characterization of many novel strains of oligotrophic marine bacteria. Strain HTCC 2207 was isolated from the Oregon coast by the HTC method. Phylogenetic analysis based on 16S rRNA gene sequence showed that this strain...
Biological di-nitrogen (N₂) fixation is a key process in open-ocean ecosystems, where the new nitrogen (N) provided by marine diazotrophs can support a large fraction of primary productivity and carbon (C) drawdown. Recent laboratory studies have shown that elevated pCO₂ enhances the rate of N₂ fixation by select laboratory isolates...
The objective of this project was to use ribosomal RNA genes, cloned
from natural populations of Sargasso Sea bacterioplankton, as markers for
picoplankton diversity. It is widely recognised that a majority of
microorganisms have yet to be cultivated, and therefore much of extant
microbial diversity remains unknown (50). The method...
Marine bacterioplankton play an important role in global elemental cycles because they return carbon dioxide and nutrients to the biosphere as they reduce organic matter. Furthermore, marine bacterioplankton are not uniformly active, and subpopulations of the in situ community may be more or less active at any given time. Defining...
A new high-throughput culturing (HTC) method using a low nutrient
heterotrophic medium (LNHM) has led to the isolation of many novel strains of
oligotrophic bacteria from marine ecosystems. Four strains belonging to a single
dade, HTCC2151, HTCC218OT, HTCC2178T and HTCC2188T, were isolated
from the coast of Oregon by the HTC...
Candidatus Pelagibacter ubique is the first cultured representative of the SAR11 clade, a clade that is found throughout the oceans and accounts for approximately 25% of all bacterial cells [1]. It has a streamlined genome that is the smallest of any known free-living organism. In this study the complete genome...
A series of experiments was performed to determine the feasibility of genomic
DNA-based methods for ecological and physiological studies of dominant bacteria from
complex natural systems. Two approaches, bacterial chromosomal painting (BCP) and
environmental genomics (EG), were evaluated for the potential to identify and enumerate
specific bacterial groups in situ...
Over 100 monthly bacterioplankton DNA samples, from each of the surface and 200 m depths at the Bermuda Atlantic Time-series Study (BATS) site, were analyzed for community assembly processes. Correlation networks, filtered for potential autocorrelation artifacts, were constructed for each depth. Network characteristics for the two depths were remarkably similar...