Antisense morpholino oligomers are synthetic macromolecules can modify gene expression. While these compounds have great potential to treat a broad range of human diseases, they suffer from poor cellular delivery. Conjugating cell-penetrating peptides onto these morpholinos is one option of addressing delivery, however development remains slow due to the cost...
Morpholino oligos (Morpholinos) are widely used tools for knocking down gene expression and are currently in a clinical trial for treatment of Duchene muscular dystrophy. A Morpholino analog has been in a clinical trial as a potential anti-bioterrorism agent for inhibiting replication of deadly Marburg viral infection. The cellular uptake...
Foundation species are important components of ecosystems because they provide habitat and ameliorate stressful conditions for residents. This thesis considers the role of surfgrasses (Phyllospadix spp.) as dynamic foundation species on the coast of Oregon in two studies. Chapter 2, which presents an observational survey of two Phyllospadix congeners, investigates...
Developing new wastewater treatment technologies which will off-set the high-energy cost associated with treatment is necessary to maintain both water and energy security. Microbial fuel cell technology represents one such option. However, there are still many obstacles to overcome before practical application of this technology can be realized. Improving cathode...
Antisense oligonucleotide (AO)-mediated exon-skipping therapeutics shows great promise in correcting frame-disrupting mutations in the DMD gene for Duchenne muscular dystrophy. However, insufficient systemic delivery limits clinical adoption. Previously, we showed that a glucose/fructose mixture augmented AO delivery to muscle in mdx mice. Here, we evaluated if fructose alone could enhance...
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jugates were kindly synthesized by Dr HongMMoulton
(Oregon State University, Corvallis, OR). PNA AOs
Antisense oligonucleotide (AO)-mediated exon-skipping therapeutics shows great promise in correcting frame-disrupting mutations in the DMD gene for Duchenne muscular dystrophy. However, insufficient systemic delivery limits clinical adoption. Previously, we showed that a glucose/fructose mixture augmented AO delivery to muscle in mdx mice. Here, we evaluated if fructose alone could enhance...
Full Text:
expression in mdx mice treated with
PMO in different concentrations of fructose (scale bar=100 μm). (b
Antisense oligonucleotide (AO)-mediated exon-skipping therapeutics shows great promise in correcting frame-disrupting mutations in the DMD gene for Duchenne muscular dystrophy. However, insufficient systemic delivery limits clinical adoption. Previously, we showed that a glucose/fructose mixture augmented AO delivery to muscle in mdx mice. Here, we evaluated if fructose alone could enhance...
Pure glycerol and the crude waste glycerin byproduct of biodiesel production were tested as substrates for electricity production in single-chamber, air-cathode microbial fuel cells (MFCs) and in single-chamber microbial electrolysis cells (MECs), using pure and mixed microbial cultures as anode biocatalyst. Current densities of 0.40 A/m² and 0.13 A/m² were...
While water scarcity and energy demand are continuously increasing in the world, alternative sources are needed to meet the requirement of a growing population. Microbial Fuel Cell (MFC) is a sustainable technology that converts organic matter in wastewater into electricity, thus it can be a potential alternative source for water...
The omnivorous ground beetle, Pterostichus melanarius Illager (Coleoptera: Carabidae), is a ubiquitous inhabitant of western Oregon crop land. Because it is often the most abundant ground beetle, and attains densities that could lead to serious impacts on its prey sources, I sought to understand the overall diet composition, and specifically...