The dataset here is used as a document file for presenting supplementary videos in the dissertation Tao, K. (2018). Investigating protein-lipid-membrane interactions in Plant Cells using Bimolecular Fluorescence Complementation (Doctoral dissertation).
Keywords: Protein, Lipid, Membrane, Plant Cells, Fluorescent Imaging
The overall focus of this thesis is on the distribution of specific lipids and membrane proteins of the external and internal membranes of plant cells, in the context of the roles that those lipids and proteins may play in microbe-plant interactions. The work includes the development of several new tools,...
WLBU2 is an engineered cationic amphiphilic peptide that targets Gram-positive and Gram-negative bacteria, and envelopes endotoxin while avoiding other cell types. The exact mechanism of how WLBU2 targets, binds, and disrupts bacterial cell membranes is still not completely known. Thus, the overall goal of this investigation is to determine the...
Abies lasiocarpa (Hook.) Nutt. (sub-alpine fir) trees of comparable
vigor growing in eastern British Columbia were studied in
order to determine the effects of cork bark disease on phellogen and
vascular cambium activity.
Diseased trees form hard deeply fissured rhytidome several
inches thick over large areas of the bole when...
Under defined cultural conditions, large populations of zygotes
of the brown algae Fucus vesiculosus L. and F. distichus L. Powell
synchronously form a cell wall shortly after fertilization. Intact cell
walls, free of cytoplasm and maintaining shape properties of early
stages of embryo development, have been isolated from these zygotes....
This work is an attempt to develop a simple physical description for the initial
interactions between a bacterial cell and a substrate surface. This is done by
adopting a view that regards bacterial surface biomolecules not as secondary and
add-on contributors to the potential energy function that characterizes the
overall...
Fucus distichus L. Powell zygotes were used to investigate
cell wall synthesis and development and to construct a model of
cell wall structure.
Alginate synthesis was investigated. Zygotes were cultured
in seawater containing ¹⁴C-bicarbonate. Alginate represented
12%, 37%, and 1.7% of the label recovered from whole zygotes,
wall, and cytoplasm,...