Vibrio cholerae, an etiological agent of cholera, circulates between aquatic reservoirs and the human gastrointestinal tract. The
type II secretion (T2S) system plays a pivotal role in both stages of the lifestyle by exporting multiple proteins, including cholera
toxin. Here, we studied the kinetics of expression of genes encoding the...
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RyanS. Simmons,b Bo R. Park,a Mariko Nonogaki,a* Sarah Emerson,c Aleksandra E. Sikoraa,b
Department
Vibrio cholerae, an etiological agent of cholera, circulates between aquatic reservoirs and the human gastrointestinal tract. The
type II secretion (T2S) system plays a pivotal role in both stages of the lifestyle by exporting multiple proteins, including cholera
toxin. Here, we studied the kinetics of expression of genes encoding the...
The bedrock of Thetis, Kuper, and seven adjacent islands and
islets of British Columbia's Gulf Islands consists of four Late Cretaceous
(Campanian) formations of the Nanaimo Group. The four, from
oldest to youngest, are the Cedar District, De Courcy, Northumberland,
and Geoffrey Formations.
The formations vary greatly in thickness but...
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OF
MICHAEL LEE SIMMONS for the MASTER OF SCIENCE
(Name) (Degree)
presented on I /'Z'
t9 (]ate
This thesis consists of three manuscripts addressing separate components of barley breeding, plus an introduction and a conclusion summarizing the status of barley in the Pacific Northwest, plus the implications of the research presented in this thesis. In the first manuscript, a package written for the statistical software "R" that...
Studies were conducted to assess influencing the effectiveness of sheep for grazing control of false brome (Brachypodium sylvaticum) an invasive grass species. Orphan lambs were exposed to false brome via grass extract in their milk and naturally reared lambs were exposed by grazing dense stands of false brome with their...
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CONTROL OF AN INVASIVE BUNCHGRASS
BY:
RYAN SCHOLZ
A THESIS
submitted to
Oregon State
The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently unknown. Here, we found that...
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Nitrogen-Rich Dissolved
Organic Matter Metabolism and C1 Compound Oxidation
Vincent J. Denef,a RyanS
This Practical Guide is a companion to the video program Communication Strategies for Fire Management: Creating Effective Citizen-Agency Partnerships. The video is designed to assist land management personnel in working collaboratively with citizens for community fi re and fuel reduction strategies. The DVD uses real world examples from successful agency...
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Ryan Gordon
Department of Forest Resources
Oregon State University
A companion to the video program
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
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reaction to label proteins?
1. Synthesize 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe) the UAA
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
This project deals with a method to optimize in vivo labeling using small fluorescent molecules via bioorthogonal reactions. The reaction used involves our unnatural amino acid 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe). The amino acid is site-specifically incorporated into GFP and then reacted with a labeled, strained trans-cyclooctene, resulting in a labeled protein. However...
Full Text:
reaction to label proteins?
1. Synthesize 4-(6-methyl-s-tetrazin-3-yl)aminophenylalanine (AMT-Phe) the UAA