Hop tissue (Humulus lupulus L.) was grown in vitro in
callus, suspension, and protoplast cultures. Medium
components were evaluated for optimal callus growth.
Solid Murashige and Skoog (MS) medium with picloram at 1.5
uM, 6-benzylaminopurine (BAP) at 10 uM, 1 g/1 casein
hydrolysate, and 100 mg/1 citric acid induced white,...
Dietary protein intake has been observed to influence the induction
of cancer in animals exposed to carcinogens, presumably by affecting the
activities of enzyme systems which metabolize them. One such carcinogen
is aflatoxin B₁ (AFB₁) a mold metabolite and frequent contaminant of
foodstuffs. Variations in the levels of dietary proteins...
Triplicate groups of rainbow trout (Salmo gairdneri) fed six
diets; five based on fish protein concentrate (FPC) ranging in caloric
density from 4.36 to 6.19 kcal/g, and one based on vitamin free
casein with a caloric density of 5.26 kcal/g were evaluated with
respect to diet ingredient utilization. The parameters...
Selected parameters that affect crude toxin production in both
primary culture (growing cell culture) and resting culture were
examined during efforts to produce sufficient crude toxin for purification.
The parameters included carbohydrate and label source and
concentration, cell concentration, incubation time and temperature
and shaker speed. A higher yield of...
Factors limiting effective extraction and purification of bean
yellow mosaic virus (BYMV) were investigated. Since the use of published
techniques for purifying BYMV resulted in low yields of partially
purified virus, an improved method for partial purification of this
virus was developed. The following treatments of infectious crude extract
decreased...