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Structure and proposed mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae

Creator:: Elkhal, Callia K., Kean, Kelsey M., Parsonage, Derek, Maenpuen, Somchart, Chaiyen, Pimchai, Claiborne, Al, and Karplus, P. Andrew
Abstract:: The formation of hydrogen peroxide (H₂O₂) by the FAD-dependent α-glycerophosphate oxidase (GlpO), is important for the pathogenesis of Streptococcus pneumoniae and Mycoplasma pneumoniae. The structurally known GlpO from Streptococcus sp. (SspGlpO) is similar to the pneumococcal protein (SpGlpO) and provides a guide for drug design against that target. However, M....
Resource Type:: Article
Full Text:: 1 Structure and proposed mechanism of α

Kinetic Mechanism of L-α-Glycerophosphate Oxidase from Mycoplasma pneumoniae

Creator:: Maenpuen, Somchart, Watthaisong, Pratchaya, Supon, Pacharee, Sucharitakul, Jeerus, Parsonage, Derek, Karplus, P. Andrew, Claiborne, Al, and Chaiyen, Pimchai
Abstract:: L-α-glycerophosphate oxidase is an FAD-dependent enzyme that catalyzes the oxidation of L-α-glycerophosphate (Glp) by molecular oxygen to generate dihydroxyacetone phosphate (DHAP) and hydrogen peroxide (H₂O₂). The catalytic properties of the recombinant His₆-GlpO from Mycoplasma pneumoniae (His₆-MpGlpO) were investigated with transient and steady-state kinetics and ligand binding. The results indicate that...
Resource Type:: Article
Full Text:: -Glycerophosphate Oxidase from 1 Mycoplasma pneumoniae 2 3 Somchart Maenpuen1, Pratchaya Watthaisong1

The Crystal Structure of the Potential Drug Target Mycoplasma pneumoniae Glycerol 3-Phosphate Oxidase

Creator:: Palioca, Callia K.
Abstract:: Mycoplasma pneumoniae is the primary cause of community-acquired pneumonias, including what is commonly known as walking pneumonia. This disease affects people from all demographics, but especially children and older adults. Outbreaks are a significant public health concern and work to develop new pharmacological agents is currently being researched. How M....
Resource Type:: Honors College Thesis
Full Text:: Crystal Structure of the Potential Drug Target Mycoplasma pneumoniae Glycerol 3-Phosphate Oxidase

Structural Insights into Novel Sugar Phosphate Cyclases and Flavoenzymes

Creator:: Kean, Kelsey M.
Abstract:: The function of a protein is defined by its three-dimensional structure, and in understanding the three-dimensional structure of a protein, we gain an understanding of its function and mechanism. Protein structures, especially at high resolution, can provide detailed insights into many elements of enzyme function and catalysis – identifying residues...
Resource Type:: Dissertation
Full Text:: ................................................................................................. 94 Chapter 5: Structure and proposed mechanism of α-glycerophosphate oxidase from Mycoplasma

Biophysical analyses of peroxiredoxins and a partner reductase

Creator:: Hall, Andrea Renée
Abstract:: Peroxiredoxins (Prxs) are dominant peroxide-reducing enzymes with two important roles: they protect all organisms from oxidative damage induced by peroxides, and in eukaryotes, they participate in hydrogen peroxide signaling pathways. This dissertation presents studies aimed at the biophysical characterization of select Prxs and a Prx reductase to elucidate their structure-function...
Resource Type:: Dissertation
Full Text:: characterization of select Prxs and a Prx reductase to elucidate their structure-function relationships. It

Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

Creator:: Perkins, Arden
Abstract:: Peroxiredoxin (Prx) enzymes catalyze the reduction of hydrogen peroxide, peroxynitrous acid, and organic peroxides, and are extremely efficient peroxidases, with k[subscript cat]/K[subscript M] on the order of 10⁷ - 10⁸ M⁻¹ s⁻¹. Besides their role in oxidative stress defense, evidence has accumulated that some eukaryotes, including humans, use Prxs as...
Resource Type:: Dissertation
Full Text:: ) assess the impact of modifications on structure and dynamics (Chapter 4) and define the roles of highly

Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

Creator:: Blanchard, David, L.
Abstract:: L-pipecolic acid (L-PA) is the higher homolog of proline. It occurs naturally in many organisms, including primates, as an intermediate in lysine degradation. The pathway by which lysine is converted into L-pipecolic acid employs the enzyme Lpipecolate oxidase (L-PO), and appears to be tissue specific to the central nervous system...
Resource Type:: Dissertation
Full Text:: ......................................................................1 I. Mechanistic Probes of L-Pipecolate Oxidase Background and Introduction

Crossing Boundaries during Stress Responses: Antigen Presentation by Adaptive and Innate MHC Class I Molecules

Creator:: Nagamine, Brandy S.
Abstract:: The immune system is a complex network of cellular processes working in harmony to guard us against pathogenic attack. Of utmost importance is the ability of the immune system to discriminate between “self” and “non-self” and diseased versus healthy. Recognition of antigens complexed to major histocompatibility complex (MHC) molecules is...
Resource Type:: Dissertation
Full Text:: from microbes, host cells, and the environment. The most well characterized of the NLRs is NOD1 and

Drug Discovery and Chemical Ecology Investigations of Specialized Metabolism in Tolypocladium and Paraisaria

Creator:: Tehan, Richard M.
Abstract:: Since the discovery of penicillin in 1928, the fungal kingdom has remained a fertile source for the discovery of new, biologically active, natural products. Further, as natural products continue to be discovered from fungi, a deeper understanding of the critical role of specialized metabolism in fungal ecology is afforded. The...
Resource Type:: Dissertation
Full Text:: Paraisaria. Traditional drug discovery methods were used toward the isolation and structure elucidation of

Transcriptional regulation of the human cathelicidin antimicrobial peptide gene

Creator:: Guo, Chunxiao
Abstract:: Human cathelicidin antimicrobial peptide (CAMP/LL-37) is a cationic antimicrobial peptide that is widely expressed by myeloid and epithelial cells at the human-environment interface. It possesses broad spectrum antimicrobial capacity against bacteria, fungi and viruses. In addition to its direct antimicrobial activity, CAMP/LL-37 also attracts and recruits monocytes, neutrophils and other...
Resource Type:: Dissertation
Full Text:: gene in U937 and HaCaT cells in presence of 1,25(OH)2D3. Results from these two chapters indicate

ScholarsArchive@OSU

Structure and proposed mechanism of L-α-glycerophosphate oxidase from Mycoplasma pneumoniae

Kinetic Mechanism of L-α-Glycerophosphate Oxidase from Mycoplasma pneumoniae

The Crystal Structure of the Potential Drug Target Mycoplasma pneumoniae Glycerol 3-Phosphate Oxidase

Structural Insights into Novel Sugar Phosphate Cyclases and Flavoenzymes

Biophysical analyses of peroxiredoxins and a partner reductase

Model systems for structural investigations into peroxiredoxin catalysis, conformation change, and inactivation

Advanced studies on cyclic amino acids in neurological signaling and peptide antibiotics

Crossing Boundaries during Stress Responses: Antigen Presentation by Adaptive and Innate MHC Class I Molecules

Drug Discovery and Chemical Ecology Investigations of Specialized Metabolism in Tolypocladium and Paraisaria

Transcriptional regulation of the human cathelicidin antimicrobial peptide gene

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