I examined, in three separate studies, the active chromatin
structure of the yeast, Saccharomyces cerevisiae.
Yeast contains four proteins having amino acid compositions
typical of the high mobility group (HMG) proteins. Three of these
are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted
by 0.25...
It has been suggested that cellular damage from oxygen radicals is one of the processes leading to cardio-vascular disease and cancer. Natural antioxidants prevent uncontrolled oxidative reactions by decreasing molecular oxygen levels, scavenging chain-initiating and chain-propagating free radicals, chelating metals, or decomposing peroxides. Beer is rich in antioxidants, derived both...
An enzyme system from cell-free yeast homogenates mediating the
transfer of the methyl group from S-adenosylmethionine-methyl-C¹⁴
or methionine-methyl-C¹⁴ to C-28 of ergosterol has been studied.
The effects of divalent cations, suithydryl inhibitors and reducing
substances on the activity of the enzyme system have been investigated.
It was established that for...
Synthesis of total RNA, DNA, and protein was followed in a
haploid yeast auxotroph during starvation for required amino acids
and uracil, and during shift-up and shift-down conditions.
During amino acid starvation, synthesis of macromolecular
constituents was not immediately affected, reflecting the presence
of large intracellular amino acid pools. Under...
The ability of cholestanol to support growth of yeast sterol
auxotrophs has been examined. Growth on this stanol was precluded
unless minute quantities of ergosterol were available, a phenomenon
designated sparking. The low levels of ergosterol were
insufficient to support growth alone. The data indicate that
cholestanol is fulfilling a...