Monoclonal antibodies were developed against USDA
challenge strain of infectious laryngotracheitis virus (ILTV).
Indirect immunofluorescence test was used to detect antibodies
in supernatants of hybridomas. Hybridoma cells were developed
by fusing Sp 2/0 myeloma cells with spleen cells obtained from
mice immunized four times with partially purified USDA
challenge strain...
Monoclonal antibodies (MCA) produced against infectious
laryngotracheitis virus (ILTV) of chickens reacted in
western blotting experiments with several different ILTV
protein bands in the absence of tunicamycin which inhibits
carbohydrate synthesis. Most of the MCA lost their
reactivity in western blotting experiments when extracts of
tunicamycin-treated ILTV CELC were used,...