Conversion of exogenous cis-zeatin to trans-zeatin in immature seeds of
Phaseolus vulgaris L. led to the isolation of a cis-trans isomerase from the endosperm.
The enzyme was purified over 2000-fold by chromatography on a series of FPLC
(anion exchange, gel filtration, and hydrophobic interaction) and Concanavalin A
columns. Non-enzymatic isomerization...