The effects of five cytokinin antagonists on the growth
of cytokinin-autonomous lines of Phaseolus lunatus cv.
Kingston, Phaseolus vulgaris cv. Great Northern, and
Nicotiana tabacum cv. Wisconsin 38 callus tissues have been
compared. The antagonists tested included four N⁴-substituted 4-amino-2-methylthiopyrrolo[2,3-d]pyrimidines
bearing the following N⁴ side chains, 4-n-pentyl-
(ms²pnN⁴Prp), 4-n-hexyl- (ms²HxN⁴Prp),...
1-Phenyl-3-(2-Thiazoly1)-2-Thiourea (PTTU), has been reported to
inhibit dopamine-fi-hydroxylase (a copper-containing enzyme involved in
the pathway of biosynthesis of adrenaline in animal systems). In the
present study, PTTU has been shown to have cytokinin activity in the
tobacco bioassay. The cytokinin activity of PTTU suggested that
cytokinin-active N⁶-substituted adenine derivatives might...
The ability of N,N'-diphenylurea (DPU) to substitute for cytokininactive
adenine derivatives in promoting callus growth of Phaseolus lunatus
and Nicotiana tabacum has been examined. In general, DPU stimulated
callus growth of P. lunatus at high concentrations and the growth of
most callus tissues was irregular, while the response of tobacco...
Cytokinin oxidase activity in callus tissues of
Phaseolus vulgaris L. cv. Great Northern has been examined
using an assay based on the oxidation of radioactively
labeled N⁶ -(Δ² -isopentenyl) adenine (i⁶Ade) to adenine
(Ade). Conditions for the quantitative extraction and
assay of the enzyme have been established. The substrate
The objective of this study was to identify the varying effects of altering the rumen fluid: substrate ratio on the outcomes of in vitro rumen fermentation. In vitro fermentation is a useful tool for evaluating the energy value of feedstuffs and the effect of feed additives on Volatile Fatty Acid...