Pseudomonas butanovora, Mycobacterium vaccae, and Nocardioides sp. CF8 utilize distinctly different butane monooxygenases (BMOs) to initiate degradation of recalcitrant chlorinated ethenes (CEs) that pollute aquifers and soils. BMO-dependent degradation of CEs such as trichloroethylene (TCE) can lead to cellular toxicities. The type and severity of TCE transformation-dependent damage can have...
Butane-grown Pseudomonas butanovora oxidized butane by a soluble
butane monooxygenase through the terminal pathway yielding 1 -butanol as the
predominant product. Alcohol dehydrogenases (ADHs) involved in butane
oxidation in P. butanovora were purified and characterized at the biochemical,
genetic and physiological levels. Butane-grown P. butanovora expressed a type I
Butane-grown cells of Pseudomonas butanovora, Nocardioides sp. CF8 and
Mycobacterium vaccae JOB5 were tested for their ability to cooxidize methane,
ammonia and ethylene. Less than 10 nmol of methane were degraded by each of
the bacteria (0.17-0.35 mg protein) in 30 minutes. Hydroxylamine and nitrite
accumulated when Nocardioides CF8 and...
The ferritin-like superfamily (FLSF) of proteins is composed of a wide variety of functionally diverse proteins involved in oxygen dependent metal-mediated electron transfer reactions. Their biological importance is exemplified by the fact FLSF proteins are found in almost every organism from all three domains of life. Their functions range from...
Iron is absolutely necessary for the maintenance, the defense, the differentiation and
last, but by no means least, the growth and cellular division of almost all living
organisms including Nitrosomonas europaea, an ammonia oxidizing bacterium. Given
the necessity of iron, it is not surprising that N. europaea evolved several irondependent...