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Bacterial Resistance to Antisense Peptide-Phosphorodiamidate Morpholino Oligomers

Creator:: Puckett, Susan E., Reese, Kaleb A., Mitev, Georgi M., Mullen, Valerie, Johnson, Rudd C., Pomraning, Kyle R., Mellbye, Brett L., Tilley, Lucas D., Iversen, Patrick L., Freitag, Michael, and Geller, Bruce L.
Abstract:: Peptide phosphorodiamidate morpholino oligomers (PPMO) are synthetic DNA mimics that bind complementary RNA and inhibit bacterial gene expression. (RFF)₃RXB- AcpP PPMO (R, arginine; F, phenylalanine; X, 6-aminohexanoic acid; B, β-alanine) is complementary to 11 bases of the essential gene acpP (encodes acyl carrier protein). The MIC of (RFF)₃RXB-AcpP was 2.5...
Resource Type:: Article
Full Text:: Morpholino Oligomer in Escherichia coli 1 Bacterial Resistance to Antisense Peptide-Phosphorodiamidate

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Creator:: Kaller, Margo
Abstract:: Peptide-phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA mimics that bind and silence gene targets. Through designing PPMOs that silence essential or resistance genes in pathogens, these antisense oligomers could be utilized as novel antimicrobials. Towards this end, my thesis employed minimum inhibitory concentration assays (MICs) to identify PPMOs capable of...
Resource Type:: Masters Thesis
Full Text:: : Evaluation of Peptide Phosphorodiamidate Morpholino Oligomers as a Novel Antibiotic Therapy. Abstract

Antisense Antibiotics

Creator:: Mitev, Georgi Mitko
Abstract:: Two different types of Phosphorodiamidate Morpholino Oligomers (PMOs) were used to inhibit growth in pure cultures of Salmonella enterica serovar typhimurium and in mouse macrophages infected with S. typhimurium. Both PMOs were 11 bases long and complementary to bases 6-16 of acyl carrier protein mRNA. A membrane-penetrating peptide, (RXR)4XB, was...
Resource Type:: Honors College Thesis
Full Text:: antisense peptide-phosphorodiamidate morpholino oligomer affect potency against Escherichia coli in vitro

Gene-specific antibiotic development: Evaluating effectiveness and investigating antibiotic-resistant Escherichia coli mutants of phosphorodiamidate morpholino oligomers

Creator:: Puckett, Susan E.
Abstract:: Phosphorodiamidate morpholino oligomers (PMOs) are novel antisense drugs in the early stages of development. These synthetic DNA mimics contain the same bases found in DNA and anneal to RNA in a complementary fashion. PMOs have been designed that target genes responsible for producing essential proteins in organisms such as Escherichia...
Resource Type:: Honors College Thesis
Full Text:: “Antisense peptide-phosphorodiamidate morpholino oligomer conjugate: dose response in mice infected with

Gene-silencing antisense oligomers inhibit Acinetobacter growth in vitro and in vivo

Creator:: Geller, Bruce L., Marshall-Batty, Kimberly, Schnell, Frederick J., McKnight, Mattie M., Iversen, Patrick L., and Greenberg, David E.
Abstract:: Background: Peptide-conjugated phosphorodiamidate morpholino oligomers (PPMOs) are synthetic DNA/RNA analogs that silence expression of specific genes. We studied whether PPMOs targeted to essential genes in Acinetobacter lwoffii and A. baumannii are active in vitro and in vivo. Methods: PPMOs were evaluated in vitro using MIC and viability assays, and in...
Resource Type:: Article
Full Text:: S-B, Weller DD. Resistance of 362 morpholino phosphorodiamidate oligomers to enzymatic degradation

In Vivo Delivery of Morpholino Oligos by Cell-Penetrating Peptides

Creator:: Moulton, Hong M.
Abstract:: Morpholino oligos (Morpholinos) are widely used tools for knocking down gene expression and are currently in a clinical trial for treatment of Duchene muscular dystrophy. A Morpholino analog has been in a clinical trial as a potential anti-bioterrorism agent for inhibiting replication of deadly Marburg viral infection. The cellular uptake...
Resource Type:: Article
Full Text:: . Weller, S.B. Huang, D.D. Weller, Resistance of morpholino phosphorodiamidate oligomers to enzymatic

Assessing an Adult Zebrafish Model for In Vivo Delivery of Morpholino Oligomers

Creator:: Kim, Joseph Y.
Abstract:: Antisense morpholino oligomers are synthetic macromolecules can modify gene expression. While these compounds have great potential to treat a broad range of human diseases, they suffer from poor cellular delivery. Conjugating cell-penetrating peptides onto these morpholinos is one option of addressing delivery, however development remains slow due to the cost...
Resource Type:: Honors College Thesis
Full Text:: phosphorodiamidate oligomers to enzymatic degradation. Antisense Nucleic Acid Drug Dev 1996; 6: 267-72. 4

CaoFructosePromotesUptake.pdf

Creator:: Cao, Limin, Han, Gang, Lin, Caorui, Gu, Ben, Gao, Xianjun, Moulton, Hong M, Seow, Yiqi, and Yin, HaiFang
Abstract:: Antisense oligonucleotide (AO)-mediated exon-skipping therapeutics shows great promise in correcting frame-disrupting mutations in the DMD gene for Duchenne muscular dystrophy. However, insufficient systemic delivery limits clinical adoption. Previously, we showed that a glucose/fructose mixture augmented AO delivery to muscle in mdx mice. Here, we evaluated if fructose alone could enhance...
Full Text:: as phos- phorodiamidate morpholino oligomer (PMO), peptide nucleic acid (PNA), 2’O-methyl

Investigations of regulatory T cell induction by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin during a graft-versus-host response

Creator:: Marshall, Nikki B.
Abstract:: The immune toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been studied for over 35 years, but only recently has the profound immune suppression associated with TCDD exposure been linked to induction of regulatory T cells (Tregs). The effects of TCDD are mediated through binding the aryl hydrocarbon receptor (AhR), a ligand-activated transcription...
Resource Type:: Dissertation
Full Text:: the TCDD-induced Tregs was IL-10. To inhibit IL-10 expression, a phosphorodiamidate morpholino

Investigation of cis-acting RNA element role in bovine viral diarrhea virus replication

Creator:: Ly, David
Abstract:: Bovine viral diarrhea virus (BVDV) is an enveloped, single-stranded positive-sense RNA virus. The BVDV genome is about 12.5 kb long and consists of a single open reading frame (ORF) flanked by untranslated regions at the 5' and 3' ends (5'-UTR and 3'- UTR, respectively). To investigate the role of cis-sequences...
Resource Type:: Masters Thesis
Full Text:: -conjugated phosphorodiamidate morpholino oligomers (PPMO) that are complementary to nucleotides (nt) 10-33

ScholarsArchive@OSU

Bacterial Resistance to Antisense Peptide-Phosphorodiamidate Morpholino Oligomers

Evaluation of peptide phosphorodiamidate morpholino oligomers as a novel antibiotic therapy

Antisense Antibiotics

Gene-specific antibiotic development: Evaluating effectiveness and investigating antibiotic-resistant Escherichia coli mutants of phosphorodiamidate morpholino oligomers

Gene-silencing antisense oligomers inhibit Acinetobacter growth in vitro and in vivo

In Vivo Delivery of Morpholino Oligos by Cell-Penetrating Peptides

Assessing an Adult Zebrafish Model for In Vivo Delivery of Morpholino Oligomers

CaoFructosePromotesUptake.pdf

Investigations of regulatory T cell induction by 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin during a graft-versus-host response

Investigation of cis-acting RNA element role in bovine viral diarrhea virus replication

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