DNA from the human squamous carcinoma cell line, SK-MES-1, and the
plasmids, pUC EJ6.6 containing H-ras oncogene, pSVc-mycl containing
cellular myc gene, pNRSac containing N-ras oncogene, pBR SV containing
SV40 virus DNA, pSV2-neu containing neu oncogene and pSV2-neo
containing neomycin resistant gene were used to transform Balb serum-free
Mouse embryo cells cultured in vitro in serum-supplemented media undergo growth
crisis, resulting in the loss of genomically normal cells prior to the appearance of
established, aneuploid cell lines. I used the technique of serum-free cell culture to develop
a serum-free mouse embryo (SFME) cell line in which serum was...
Cell culture systems have provided many insights into
eukaryotic gene expression and other biochemical
mechanisms. Since the cell represents the smallest living
unit of any organism it provides a desirable in vitro
system, allowing biochemical studies without the complex
physiology of an entire animal. However, processes
involving intracellular mechanisms, such...
Serum-free mouse embryo (SFME) cells are derived
in medium in which serum is replaced with growth
factors and other supplements. They display unusual
properties. They do not lose proliferative potential
or show gross chromosomal aberration upon extended
culture, they depend on epidermal growth factor (EGF)
for survival, and are reversibly...
Serum-free mouse embryo (SFME) cells, which were derived
from 16-day-old Balb/c mouse embryo brain, grow in absence of
serum without losing genomic normality or proliferative
potential, and require epidermal growth factor (EGF) for
normal growth. EGF is a well studied mitogen that binds to a
specific receptor on the cell...
Rainbow trout cystatin C cDNA has been isolated from
trout liver. The full-length cystatin cDNA (674 bp)
included the 5'untranslated region and the polyadenylation
signal sequence AATAAA in the 3' region. Translation of
the cDNA defines 132 amino acid residues. Comparison of
the amino acid sequence with those of family...
The thesis area comprises 53 square miles located in Beaverhead County, Montana, and includes the Argenta mining district. Sedimentary and igneous rocks of the area range in age from Precambrian to Recent. The aggregate thickness of the sedimentary rocks is approximately 13,500 feet. The only systems not represented are Ordovician...
The Supplemental Poverty Measure (SPM) is the newest method to paint a more accurate picture of the scope of poverty in the United States than the Official Poverty Measure (OPM). In doing so, the SPM includes many sources of income, including income from work and in-kind government supplemental policies. The...