Index Catalog // ScholarsArchive@OSU

Ion pairing of nucleotides with surfactants for enhanced sensitivity in liquid matrix assisted secondary ion mass spectrometry

Creator:: Pavlovich, James Gilbert
Abstract:: In particle induced desorption-ionization mass spectrometry the strength of an analyte's signal under a given set of bombardment conditions is usually considered to be representative of the analytes relative surface activity. This rationale is generally used to explain differences in the technique's sensitivity between and within various classes of compound....
Resource Type:: Dissertation
Full Text:: Secondary Ion Mass Spectrometry Abstract approved: ouglas F. Barofsky In particle induced desorption

Ion sputtering from organic liquid matrices bombarded by keV metal ions

Creator:: Yen, Ten-Yang
Resource Type:: Dissertation
Full Text:: keV Metal Ions Abstract approved: Douglas F. Barofsky Ion sputtering from organic liquid matrices

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Creator:: Jensen, Ole Norregaard
Abstract:: A novel protocol for the study of protein-nucleic acid interactions is presented and demonstrated to be feasible. The protocol combines photochemical crosslinking techniques and mass spectrometric methods into a new strategy for identifying protein domains or amino acid residues that are in close contact with nucleic acid in protein-nucleic acid...
Resource Type:: Dissertation
Full Text:: approved: ouglas F. Barofsky A novel protocol for the study of protein-nucleic acid interactions is

Characterization of UV-crosslinked protein-nucleic acid interfaces by Maldi MS and ESI MS/MS

Creator:: Gafken, Philip R.
Resource Type:: Dissertation
Full Text:: molecular weights to over 100 kDa from sample amounts on the order of 0.1 to 10 pmol. Barofsky and co

Mass spectrometric analysis of UV-crosslinked protein-nucleic acid complexes

Creator:: Doneanu, Catalin E.
Abstract:: The DNA-binding domains of E. coli uracil-DNA glycosylase (Ung) and human replication protein A (hRPA) were studied using a general protocol developed in our laboratory for probing protein-DNA interactions. The procedure involves purification and mass spectrometric analysis of the nucleopeptide-products of a tryptically digested UV-crosslinked protein-nucleic acid complex. In the...
Resource Type:: Dissertation
Full Text:: Analysis of UV- Crosslinked Protein-Nucleic Acid Complexes Abstract approved: F. Barofsky The DNA

Determination of fluorinated alkyl substances in aqueous systems

Creator:: Schultz, Melissa M.
Abstract:: Fluorinated alkyl substances, which can be persistent, toxic, and bioaccumulative, have been quantitated in many densely populated and remote regions, including in air, surface waters, groundwater, and biota; however, little is known about their transport or behavior in the environment. Wastewater effluent is one of the principal routes for introducing...
Resource Type:: Dissertation
Full Text:: Fluorinated Alkyl Substances in Aqueous Systems. Abstract approved: Douglas F. Barofsky Fluorinated alkyl

Proteomic approach to the analysis of DNA-binding proteins using mass spectrometry

Creator:: Stapels, Martha Degen
Abstract:: In proteomic studies, separate experimental protocols have been necessary to identify proteins, determine their function, and predict their three-dimensional structure. In this study, a function-based separation of proteins was conceived to fractionate proteins prior to enzymatic digestion. In the initial demonstration of this technique, a DNA substrate was used to...
Resource Type:: Dissertation
Full Text:: Douglas F. Barofsky Reproduced with permission from Journal of Proteome Research, submitted for

Gas-phase fragmentation chemistry of protonated peptide ions

Creator:: Bythell, Benjamin James
Abstract:: The fragmentation characteristics of protonated peptides have been investigated using tandem mass spectrometry (MS/MS) under various fragmentation regimes and detailed density functional theory (DFT) reaction pathway calculations. The DFT calculations predict novel salt-bridge stabilized transition structures as well as consecutive reactions occurring in proton-bound dimers for some of the ions...
Resource Type:: Dissertation
Full Text:: Protonated Peptide Ions Abstract approved:________________________________ Douglas F. Barofsky

“SeW: A Search for Selenosulfide Bonding” and “Severity of Damage Inflicted on Intercellular Disulfide Bonds as a Result of UV Radiation”

Creator:: Dutcher, Kendall
Abstract:: Part 1: White muscle disease (WMD), a degenerative muscle disease brought on by a deficiency of the essential trace nutrient selenium, is thought to be mediated by the protein Selenoprotein W (SeW). In its relationship with WMD, SeW, like many selenoproteins, binds to glutathione, serving some purpose in redox mechanisms...
Resource Type:: Undergraduate Thesis and Research Paper

Development, validation and the application of a congener specific photodegradation model for PBDEs

Creator:: Zeng, Xia
Abstract:: The thermodynamic properties of 39 PBDEs have been calculated using Gaussian 03 on the B3LYP/6-31G(d)//B3LYP/6-31G(d) level. The PBDE congeners' enthalpies of formation increase with increasing number of bromines and show a strong dependence on the bromine substitution pattern. The effects of bromine substitution pattern have been quantitatively studied by GAM...
Resource Type:: Dissertation
Full Text:: approved: Staci L. Simonich Douglas F. Barofsky The thermodynamic properties of 39

ScholarsArchive@OSU

Ion pairing of nucleotides with surfactants for enhanced sensitivity in liquid matrix assisted secondary ion mass spectrometry

Ion sputtering from organic liquid matrices bombarded by keV metal ions

Characterization of photochemically cross-linked protein-nucleic acid complexes by mass spectrometry

Characterization of UV-crosslinked protein-nucleic acid interfaces by Maldi MS and ESI MS/MS

Mass spectrometric analysis of UV-crosslinked protein-nucleic acid complexes

Determination of fluorinated alkyl substances in aqueous systems

Proteomic approach to the analysis of DNA-binding proteins using mass spectrometry

Gas-phase fragmentation chemistry of protonated peptide ions

“SeW: A Search for Selenosulfide Bonding” and “Severity of Damage Inflicted on Intercellular Disulfide Bonds as a Result of UV Radiation”

Development, validation and the application of a congener specific photodegradation model for PBDEs

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