Pre-existing Expressed Sequence Tag (EST) libraries (1,305 sequences) and a
newly constructed microsatellite-enriched genomic library (136 clones) from the V.
corymbosum cultivar 'Bluecrop' were screened for unique Simple Sequence Repeat
(SSR)-containing sequences. Our goal was to develop a set of robust SSR markers for
use in managing the National Clonal...
This dissertation describes the application and development of molecular tools that have great potential for use in studying variation in strawberry germplasm. The first study evaluated 91 microsatellite (simple sequence repeat, SSR) markers for transferability in 22 Fragaria species and their utility in fingerprinting octoploid strawberries. Out of the transferable...
Conversion of exogenous cis-zeatin to trans-zeatin in immature seeds of
Phaseolus vulgaris L. led to the isolation of a cis-trans isomerase from the endosperm.
The enzyme was purified over 2000-fold by chromatography on a series of FPLC
(anion exchange, gel filtration, and hydrophobic interaction) and Concanavalin A
columns. Non-enzymatic isomerization...
Strawberry is economically the most important berry crop grown worldwide and breeders are continuously striving to develop improved cultivars. So far, marker assisted breeding (MAB) in strawberry has been limited to the private sector. However, loci controlling some traits of economic importance such as perpetual flowering (PF) and soluble solids...
European hazelnut is a significant crop in the Pacific Northwest, and the US ranks
4th internationally for hazelnut production. Production in the Pacific Northwest is
threatened, however, by the disease eastern filbert blight (EFB) caused by the fungus
Anisogramma anomala (Peck) E. Müller. To meet the challenges faced by the...
Strawberry is one of four crops included in the USDA-NIFA Specialty Crop Research Initiative-funded RosBREED project along with apple, peach, and cherry. Phenotyping strawberry for specific horticultural and commercial traits is important to identify associations with genotypic marker(s). This process is the first step in translating genomic knowledge into enhanced...
Twenty-one pairs of simple sequence repeat (SSR) primers were used to
investigate genetic diversity in 270 accessions of European hazelnut (Corylus avellana)
representing a wide geographic range. A capillary electrophoresis system and ABI
Genescan® and Genotyper® software were used to determine the allele size generated
from each PCR reaction based...
Two studies used DNA markers to assess their usefulness in germplasm
identification and evaluation of genetic stability in four cryopreserved Rubus
accessions that were stored for over 10 years in liquid nitrogen. In the first study, 12
robust Simple Sequence Repeat (SSR) markers were developed from two
microsatellite-enriched libraries of...
The hundred-year history of the hazelnut industry in the Pacific Northwest
is threatened by eastern filbert blight (EFB) caused by the fungus Anisogramma
anomala (Peck) E. Müller. Marker-assisted selection (MAS) has been extensively
used for ‘Gasaway’ resistance in the hazelnut breeding program at Oregon State
University. Concern over breakdown of...