RNA isolated from BAI strain A (Myeloblastosis) Avian Tumor
Virus can be fractionated into two fractions on a sucrose density
gradient, one of higher molecular weight and one of low molecular
weight. The low molecular weight (LMW) virus RNA was found to
chromatograph the same as E. coli tRNA on...
Avian myeloblastosis virus RNA was fractionated into a high
molecular weight RNA and a low molecular weight RNA fraction by
sucrose density centrifugation. The larger RNA component had a
sedimentation constant of 65S and the low molecular weight RNA
about 4S. The low molecular RNA has two biological properties of...
The genome of the nuclear polyhedrosis virus which infects the
larvae of the tussock moth, the lepidopteran, Hemerocampa
pseudotsugata, was isolated, identified as deoxyribonucleic acid, and
characterized in terms of its physical and biological properties. An
isolation procedure for the viral DNA was established which consisted
of the following steps:...
RNA tumor viruses, such as avian myeloblastosis virus (AMV)
and MC29 virus, cause malignant transformation of the cells they infect.
Transformed cells are able to continually produce progeny virus
particles.
Evidence is given that RNA-dependent RNA polymerase is present
in virus infected cells. RNA-dependent RNA polymerase isolated
from AMV-infected myeloblasts...
There is good evidence that the infection with avian myeloblastosis
virus BAI strain A causes myeloblasts to retain their primitive
nature. These cells are able to multiply and produce virus at a relatively
constant rate. From these observations, a major problem develops
in the study of the virus-induced leukemia, that...
Chicken embryonic cells (CEC) cultured in vitro were able to
withstand the toxicity of DDT at concentrations below 10μg/ml culture
medium. When the DDT concentration exceeded the toxic level, CEC
appeared vacuolated and lost the spindle-like fibroblastic appearance,
Gas chromatographic analysis had shown that the uptake of DDT (3μg/
ml'culture...
Chromatin DNA sequences intimately associated with the "core"
of the basic, repeating chromatin subunit are shown to be relatively
resistant to digestion by micrococcal nuclease. Chromatin DNA
sequences lying between adjacent chromatin subunits, as well as
DNA sequences in regions of chromatin which are free of chromatin
subunits, are subject...
The cytolytic properties of the mosquitocidal 25 Kd protein deltaendotoxin of Bacillus thuringiensis subsp. israelensis (Bti) was studied using the human erythrocyte as a target cell. Both a fixedtime and a kinetic hemolytic bioassay were developed in order to screen a variety of treatments for their influence on the toxinerythrocyte...