Sodium dodecyl sulfate polyacrylamide gel electrophoresis and
zymography were used in two separate experiments to determine the tissue
source and type of plasminogen activator (PA) produced by bovine
blastocysts. Twelve-14 d blastocysts were collected at slaughter from
estrous synchronized, superovulated and artificially inseminated Holstein
cows. In Experiment I, blastocysts were...
Although the occurrence of ketosis is a postpartum phenomenon, recent studies have
focused on the prepartum period as key in the development of the disorder. Indicators of
prepartum energy status, such as depressed dry matter intake (DMI) and elevated plasma
non-esterified fatty acid (NEFA) concentrations have been associated with the...
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Maternally-derived transcripts and proteins support early bovine and ovine embryo development until the 8- to 16-cell stage, at which time embryonic transcripts become essential for continued development. One purported mechanism for the switch from maternal to zygotic control of development (maternal to zygotic genome activation; MZGA) is the appearance of...
Day 12-14 bovine embryos produce urokinase-type plasminogen activator (uPA) and
an uPA-plasminogen activator inhibitor (PAI) complex. The type of PAI, -1 or -2, in
the complex has not been determined therefore the objective of this research was to
identify the PAI produced by the Day 14 bovine embryo. In experiment...
During early embryonic development, endodermal cells leave the inner cell mass (ICM) and migrate over an extracellular matrix (ECM), located on the blastocoelic side of the trophectoderm, to form a continuous layer of extraembryonic endoderm. Cell migration events depend on a family of cell surface proteins known as integrins that...
Three experiments compared ruminal, physiological, and performance responses of beef steers consuming hay ad libitum and receiving grain-based supplements with (CAM) or without (CO) inclusion of camelina meal. In Exp. 1, 9 steers fitted with ruminal cannulas received CAM (2.04 kg of DM/d) or CO (2.20 kg of DM/d). Steers...
The type of plasminogen activator (PA) secreted by bovine embryos was identified. Day 12 to 14 blastocysts were collected at slaughter from estrous-synchronized, superovulated and naturally mated crossbred beef cows. Embryos were either left as intact embryos (E) or microdissected into component embryonic discs (ED) and trophoblastic vesicles (TV). Intact...