Cryopreservation of adherent cells may be advantageous for cell types that are difficult to
preserve in suspension or when it is necessary to preserve characteristics of the adherent cultured cells. Vitrification is a promising procedure for the preservation of adherent cells that prevents ice crystal formation and the resulting dissociation...
Atherosclerosis is the underlying cause of ischemic heart disease and stroke and is the leading cause of death worldwide, especially in developed countries. As an inflammatory disease of arteries in a hyperlipidemic milieu, expression of adhesion molecules, such as endothelial-leukocyte adhesion molecule-1 (E-selectin) and intercellular adhesion molecule-1 (ICAM-1), on endothelial...
The goal of this dissertation was to improve our abilities in acquiring critical in vivo data by establishing embryo-larva zebrafish as an exemplary model to quantitatively evaluate cancer progression. The living environment that encompasses a tumor has a significant effect on how cancer develops, grows and metastasizes. In order to...
This work is an attempt to develop a simple physical description for the initial
interactions between a bacterial cell and a substrate surface. This is done by
adopting a view that regards bacterial surface biomolecules not as secondary and
add-on contributors to the potential energy function that characterizes the
overall...
This research details laboratory assessment of bacterial hydrophobicity, variations in bacterial adhesion properties with growth and extended starvation as well as extension of this assay to colloidal hydrophobicity measurement. Hydrophobicity is an integral component of surface interactions and has been studied extensively for its role in a number of engineering...
Clostridium perfringens is a spore-forming pathogenic bacterium that causes a variety of diseases in human and animals. C. perfringens type A isolates produce enterotoxin (CPE) causing food poisoning (FP) and non-food-borne (NFB) gastrointestinal (GI) diseases including antibiotic-associated diarrhea and sporadic diarrhea. C. perfringens type A food poisoning currently ranks as...
WLBU2 is an engineered cationic amphiphilic peptide that targets Gram-positive and Gram-negative bacteria, and envelopes endotoxin while avoiding other cell types. The exact mechanism of how WLBU2 targets, binds, and disrupts bacterial cell membranes is still not completely known. Thus, the overall goal of this investigation is to determine the...